摘要
目的考察FL-1大孔树脂吸附纯化番石榴叶总黄酮的工艺条件及参数。方法用紫外分光光度法测定番石榴叶总黄酮的含量,以树脂吸附量、上液容量体积为指标考察FL-1树脂的番石榴叶总黄酮的吸附行为。结果FL-1大孔树脂对番石榴叶总黄酮吸附纯化的最佳条件为:样品中总黄酮的浓度为13.22%,原药材与树脂投量比为1∶4,以70%乙醇作为洗脱剂、洗脱流速为2 mL/min。采用本工艺,番石榴叶总黄酮的得率达30.12%,纯度达55.22%。结论该工艺简便,适于番石榴叶总黄酮的吸附纯化。
Objective To evaluate the performance of FL-1 macroporous absorption resin for absorption of total flavonoids from Psidium guajava leaf. Methods The concentration of total flavones in Psidium guajava leaf was determined by ultraviolet spectrophotometry and the absorption behavior of FL-1 macroporous adsorption resins to total flavones in Psidium guajava leaf was examined for the adsorption capacity and the volume of solution loaded. Results Optimal absorption of total flavonoids was achieved with the sample： total flavonoids concentration in the solution of 13.22 mg/mL, the ratio of total flavonoids concentration and macroporous adsorption resin was 1 ： 4, washing with 70% ethanol at the flow velocity of 2 BV/h. Conclusion FL- 1 Macroporous absorption resin can be well applicable for enrichment of total flavonoids in Psidium gualjava leaf.
出处
《中国中医药信息杂志》
CAS
CSCD
2008年第6期56-57,共2页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(30572458)
广东省自然科学基金(07005190)
中国博士后科学基金(2004035612)
关键词
番石榴叶
总黄酮
大孔树脂
Psidium guajava leaf
total flavonoids
macroporous adsorption resins
