摘要
目的:建立高效液相色谱法测定玄麦甘桔颗粒中甘草苷、甘草酸、哈巴俄苷、肉桂酸含量。方法:采用 LiChrospher RP-C_(18)(250 mm×4.6 mm,5μm)色谱柱,以乙腈-水(含1.0%甲酸)为流动相,梯度洗脱(0～20 min,流动相比例20:80→50:50),流速1 mL·min^(-1),双波长检测[278 nm(0～18 min,测定甘草苷、哈巴俄苷及肉桂酸),254 nm(18～20 min,测定甘草酸)]。结果:甘草苷、甘草酸、哈巴俄苷和肉桂酸分别在0.21～6.30,0.25～7.50,0.10～3.00,0.105～3.15 μg范围内呈良好的线性关系;平均回收率(n=9)分别为99.6%,99.3%,100.5%,98.9%。结论:该方法简便迅速,准确可靠,重现性好,结果稳定,适合玄麦甘桔颗粒的含量控制。
Objective ： To establish an RP - HPLC method for determination of contents of glycyrrhizin, glycyrrhizic acid ,harpagoside and cinnamic acid in Xuanmai Ganjie granules. Methods：A LiChrospher RP - C18 （250 mm × 4. 6 mm,5 μm）column was adopted,the mobile phase was acetonitrile- water（contained 1.0% formic acid）for gradient elution（0 -20 min, ratio of the mobile phase was 20： 80→50： 50）. The double detection wavelengths were used [ 278 nm （0 - 18 min for glycyrrhizin, harpagoside and cinnamic acid ;254 nm （ 18 - 20 min for glycyrrhizic acid） ]. Results：Good linearities were obtained from 0.21 to 6. 30,0.25 to 7.50,0. 10 to 3.00 and 0. 105 to 3.15 μg for glycyrrhizin, glycyrrhizic acid, harpagoside and cinnamic acid respectively. The mean recoveries （ n = 9 ） of components were 99.6% ,99.3% , 100.5% and 98.9% respectively. Conclusion：The method is accurate,rapid and simple, and is suitable for the quantitative analysis and related components test of Xuanmai Ganjie granules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第5期735-738,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
高效液相色谱
甘草苷
甘草酸
哈巴俄苷
肉桂酸
HPLC
glycyrrhizin
glycyrrhizic acid
harpagoside
cinnamic acid
Xuanmai Ganjie granules
