摘要
目的:建立附子理中丸中甘草酸的反相高效液相色谱分析方法。方法:Kromasil C_(18)色谱柱(250 mm×4.6 mm,5 μm),流动相为甲醇-水-乙醚-冰醋酸(60:34:6:3),流速1.0 mL·min^(-1),检测波长250 nm,柱温(30±0.5)℃。结果:甘草酸进样量在0.4～8.0μg范围内线性关系良好,平均同收率(n=6)为97.2%(RSD=0.5%)。结论:该方法快速、准确,可用于附子理中丸中甘草酸的含量测定。
Objective：To develop an RP - HPLC method for the quantitative determination of glycyrrhizic acid in Fuzi Lizhong pills. Methods：The analysis was carried out on a Kromasil C18 column （250 mm×4.6 mm,5 μm）with the mixture of methanol- water- diethyl ether- glacial acetic acid（60： 34： 6： 3）as the mobile phase at a flow rate of 1.0 mL · min^-1 ,the detection wavelength was set at 250 nm and the column temperature was maintained at（30 ± 0.5 ） ℃. Results： The calibration curve showed a good linearity with the range of 0.4 - 8.0 μg for glycyrrhizic acid, and the mean recovery（ n = 6） was 97.2% （ RSD = 0.5% ）. Conclusion：This method is simple and accurate. And it can be used for the determination of glycyrrhizic acid in Fuzi Lizhong pills.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第5期779-781,共3页
Chinese Journal of Pharmaceutical Analysis
