摘要
目的探讨电针阻断胰岛素抵抗及过氧化反应治疗非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)的机制。方法SD大鼠33只按随机抽签法分组:正常对照组(11只),造模组(22只)。正常组以普通饲料喂养,造模组以高脂饲料喂养,8周后处死3只(正常对照组1只,造模组2只),验证造模成功后将造模组分为NAFLD模型组(10只)和电针治疗组(10只),后者施以电针治疗。12周末处死所有大鼠检测血糖(FBG)、血清胰岛素(FINS)、血清游离脂肪酸(FFA)、肝内丙二醛(MDA)、超氧化物歧化酶(SOD)、甘油三酯(TG)、总胆固醇(TC)、谷胱甘肽(GSH)含量变化及肝组织病理学。结果与模型组比较,电针组FBG、FINS、FFA降低(P<0.05);肝内MDA、TG、TC显著下降(P<0.01);SOD、GSH活性升高(P<0.01),肝组织脂肪变性和炎性损伤得以改善。结论电针通过阻止胰岛素抵抗和过氧化反应之间的恶性循环治疗NAFLD。
Objective To investigate the mechanism of electroacupuncture in the treatment of non-alcoholic fatty liver disease （NAFLD） . Methods Thirty-three male SD rats were randomized into 2 groups ： normal control group （n=11, fed with normal food） and experimental rats （n=23, fed with high-fat food ）. Eight weeks later, 3 rats were randomly selected from the 2 groups respectively and killed to undergo pathological examination of the liver. When the establishment of experimental rats was confirmed, the remaining 20 experimental rats were divided into 2 equal subgroups： NAFLD group （fed continuously with high-fat food） and electroa-cupuncture treatment group （fed continuously with high-fat food and received the treatment 15 min per day, once a day for 4 weeks）. Then all rats were killed to detect the serum levels of fasting blood glucose （FBG）, fasting insulin （FINS） and free fatty acids （ FFA）, liver contents of malondialdehyde （ MDA）, glycerin tri-palmitate （TG） and total cholesterol （TC）, and the activities of liver superoxide dismutase （SOD） and glutathione （GSH）. Liver pathological changes was examined. Results Compared with NAFLD group, electroacu- puncture reduced the serum levels of FBG, FINS and FFA （ P 〈 0.05） , the liver contents of MDA, TG and TC （ P 〈0.01 ）, and increased the activities of SOD and GSH （P 〈0. 01 ）. It also attenuated the liver fatty degen-eration and inflammatory injury. Conclusion Electroacupuncture can treat NAFLD by blocking the vicious circle of insulin resistance and perooxidation.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2008年第11期1055-1057,共3页
Journal of Third Military Medical University
关键词
非酒精性脂肪肝
胰岛素抵抗
氧化抗氧化
针灸治疗
non-alcoholic fatty liver disease
insulin resistance
oxidation antioxidation
acupuncture
