摘要
目的：观察丁基苯酞（ＮＢＰ）对大鼠局灶性脑缺血及重灌后海马，纹状体和皮层中ＴＸＢ２及６ｋｅｔｏＰＧＦ１α含量的影响．方法：尼龙线栓塞法造成大鼠局灶性脑缺血模型．ＴＸＢ２和６ｋｅｔｏＰＧＦ１α用放免法测定．结果：ＮＢＰ１０ｍｇ·ｋｇ－１治疗对缺血重灌注后脑组织中ＴＸＢ２的产生具有抑制作用，但对６ｋｅｔｏＰＧＦ１α的产生无明显作用．ＮＢＰ２０ｍｇ·ｋｇ－１治疗后，重灌５ｍｉｎ缺血脑组织中ＴＸＢ２和重灌后３０ｍｉｎ时６ｋｅｔｏＰＧＦ１α含量皆明显减少．ＮＢＰ２０或１０ｍｇ·ｋｇ－１皆明显提高ＰＧＩ２／ＴＸＡ２的比值．而阿司匹林（２０ｍｇ·ｋｇ－１）除重灌５ｍｉｎ提高纹状体ＰＧＩ２／ＴＸＡ２的比值外，在其它时间点上均无提高作用．结论：ＮＢＰ提高缺血脑组织中ＰＧＩ２／ＴＸＡ２的比值，可能有利于改善缺血脑组织的微循环状态．
IM: To study the effects of dl 3 n butylphthalide (NBP) on the changes of thromboxane B 2 (TXB 2) and 6 keto PGF 1 alpha (6 keto PGF 1α ) contents in hippocampus, striatum, and cerebral cortex of rats subjected to focal cerebral ischemia followed by reperfusion. METHODS: Focal cerebral ischemia was induced by inserting a nylon suture into intracranial segment of internal carotid artery from external carotid artery and blockade of the origin of middle cerebral artery. For reperfusion, the suture was pulled out to restore the blood flow to the ischemic brain. Determination of TXB 2 and 6 keto PGF 1α was performed by RIA method. RESULTS: Reperfusion following focal cerebral ischemia resulted in increases in TXB 2 at 5 min and 6 keto PGF 1α at 30 min and a decrease in the ratio of epoprostenol (PGI 2)/thromboxane A 2 (TXA 2) (6 keto PGF 1α /TXB 2) at 5 min in hippocampus, striatum, and cerebral cortex. NBP 10 mg·kg -1 reduced the content of TXB 2 without decreasing effect on 6 keto PGF 1α . NBP 20 mg·kg -1 reduced both TXB 2 and 6 keto PGF 1α in lesser extent than aspirin (Asp, 20 mg·kg -1 ). NBP 20 or 10 mg·kg -1 elevated the ratio of PGI 2/TXA 2 after reperfusion, but Asp 20 mg·kg -1 did not increase the ratio except in striatum at 5 min after reperfusion. CONCLUSION: NBP increases the ratio of PGI 2/TXA 2 which may have beneficial effects on the impaired microcirculation in postischemic brain tissues.
出处
《中国药理学报》
CSCD
1997年第6期505-508,共4页
Acta Pharmacologica Sinica
关键词
丁基苯酞
血栓素B2
酮前列腺素F1α
局灶
脑缺血
dl
3
n
butylphthalide
thromboxane B 2
6 ketoprostaglandin F 1 alpha
aspirin
transient cerebral ischemia
