摘要
从双酶配比、酶解pH值、酶解温度、酶解时间研究了中性蛋白酶(1398)和碱性蛋白酶(2709)对大豆分离蛋白酶解的影响,并运用正交试验设计和方差分析得到复合酶解最佳条件。单因素结果表明以双酶配比为1:3(1398:2709)、pH9.5、60℃和酶解3.5h时对水解较为有利。正交试验和方差分析表明,双酶配比(1398:2709)和pH值对酶解影响最为显著(P<0.05),酶解时间和温度对酶解影响最不显著(P>0.05)。
The influences of the ratio of neutral proteinase （1398） and alkaline proteinase （2709）, enzymolysis pH, enzymolysis temperature and enzymolysis time on soybean protein enzymolysis was studied. The optimal conditions for double-enzyme hydrolysis were determined by orthogonal test and variance analysis. Single factor tests indicated that when the ratio of enzyme 1398 to 2709 was 1：3, the hydrolysis carried out very successfully at pH 9.5 and 60℃ for 3.5 h. The results of orthogonal test and ANOVA analysis indicated that the pH and the ratio of enzyme 1398 and 2709 had significant effects on hydrolysis （P〈0.05） .while the enzymolysis time and hydrolysis temperature did not have significant effects （P〉0.05）.
出处
《中国酿造》
CAS
北大核心
2008年第6期28-30,共3页
China Brewing
基金
长沙市科技局资助项目(BK0422,K032035212)
关键词
大豆分离蛋白
水解度
中性蛋白酶
碱性蛋白酶
soybean protein isolate
degree of hydrolysis
neutral proteinase
alkaline proteinase
