摘要
为证明癫痫发作早期一氧化氮（ＮＯ）抗发作效应，用ＮＯ合酶（ＮＯＳ）抑制剂Ｌ－硝基精氨酸甲酯（Ｌ－ＮＡＭＥ）对大鼠红藻氨酸（ＫＡ）诱导性发作进行干预，同时用分光光度法检测海马结构中ＮＯＳ活性的早期变化。发现ＫＡ发作１０ｍｉｎ、３０ｍｉｎ组海马结构中ＮＯＳ活性明显升高，而ＫＡ注射前３０ｍｉｎ给予Ｌ－ＮＡＭＥ可显著抑制ＮＯＳ活性的升高，这种抑制效应与大鼠ＫＡ发作中湿狗样摇动（ＷＤＳ）的提早出现和发生次数增多显著相关。结果提示在ＫＡ诱导大鼠发作早期内源性ＮＯ具有明显的抗发作效用。
The anticonvulsant effect of endogenous nitric oxide (NO) on kainic acid (KA) induced seizures in rats was investigated.Intervention by L-nitro-arginine melthy ester (LNAME),an inhibitor of NO synthase(NOS),for KA induced seizures was conducted and the variation of NOS activity in rat hippocampal was determined spectrophtometrically.We found that KA caused overt behavioral abnormalities,such as wet dog shakes(WDS).NOS activity in rat hippocampus significantly increase 10 and 30 min after KA injection but was significantly inhibited by LNAME administration 30min prior to KA injection to a certain extent.The inhibition effect of LNAME was related to both earlier occurrence and greater numbers of WDS in KA seizures.The present results suggest that endogenous synthsis and release of NO may show a potent action of antiseizure in the early stage of KA induced seizures.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
1997年第6期340-341,共2页
Journal of Apoplexy and Nervous Diseases
关键词
红藻氨酸
一氧化氮
一氧化氮合酶
L-NAME
癫痫
Kainic acid L-nitro-arginine melthy ester Nitric oxide Nitric oxide synthase Seizure
