摘要
为探讨外生菌根的形态及根毛生长状况对宿主生长的影响,分别对黑松接种3种不同外生菌根菌进行研究,观察菌根形态,并对菌根根段进行透明染色后观察根毛的生长发育程度及菌套、哈氏网的分布状况。结果表明:黑松与不同外生菌根菌形成的菌根形态各异,黄色须腹菌(Rl)和彩色豆马勃(Pt2)的菌根以多头状分支、二叉分支、多级二叉分支为主,美味牛肝菌(Be)的菌根多见二叉分支和顶端明显膨大的棒状。菌根的形态与黑松生长状态似无明显相关性。黑松不同外生菌根根段上均可观察到根毛,且根毛的生长发育状态与菌根菌对黑松的促生作用具有一定相关性。在生长较好的Rl和Pt2菌根化松苗中具根毛的菌根段所占比例较高,且在根毛生长区可同时观察到较多哈氏网菌丝;而在Be菌根苗和对照菌根中,具根毛的菌根段相对较少,在根毛生长区很难观察到根内哈氏网,且杂菌较多。
In this paper, three ectomycorrhizal fungi were inoculated to Pinus thunbergii seedlings. The ectomycorrhizal morphologies, as well as root hair growth and mantle, hartig net distribution were observed. The morphology of different ectomycorrhizas was various. The dominant forms of ectomycorrhizas developed by Rhizopogen luteous（R1） affd Pisolithus tinctorius（Pt2） were coralloid branching, dichotomous branching, and multilevel dichotomous branching. The dominant forms of ectomycorrhizas developed by Boletus edulis（Be） were dichotomous branching and club-shaped ectomycorrhiza with a enlarged apex. The morphologies of ectomycorrhizas seemed not obviously correlated with the seedlings growth. Root hairs were observed in ectomycorrhizal short root. It showed some significant correlation with the seedlings growth. The percentage of ectomycorrhizal short roots with root hairs was higher in R1 and Pt2 than that in Be treatment. At the same time, many Hartig net hyphae were observed in the growth region of root hairs in the former, but many other fungi were observed in Be and the control treatment. This study was helpful to understand the root hairs growth and their function in the ectomycorrhizas. It was also useful to further explore the reciprocity and coevolution between the host plant and mycorrhizal fungi.
出处
《南京林业大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第3期107-111,共5页
Journal of Nanjing Forestry University：Natural Sciences Edition
基金
国家自然科学基金资助项目(30571471)
江苏省自然科学基金重点资助项目(BK2004217)
关键词
黑松
外生菌根
菌根形态
根毛
Pinus thunbergii
Ectomycorrhiza
Ectomycorrhizal morphology
Root hairs
