摘要
目的:探讨防风多糖对体外培养人白血病K562细胞有无调亡诱导作用。方法:MTT法检测防风多糖对K562细胞的增殖抑制作用;荧光显微镜观察细胞的形态学变化;琼脂糖凝胶电泳分析细胞凋亡的DNA断裂;流式细胞术采用Annexin-V/PI双染实验检测细胞凋亡。结果:MTT法检测显示防风多糖对K562细胞具有显著生长抑制作用;荧光显微镜下观察发现防风多糖作用的K562细胞中出现核固缩、凋亡小体;琼脂糖凝胶电泳呈现DNA梯形条带即(DNA ladder);流式细胞仪分析结果显示细胞凋亡百分率随防风多糖浓度增加而增加。结论:防风多糖对体外培养人白血病K562细胞具有增殖抑制及凋亡作用。
Objective：To investigate whether the Fangfeng Polysaccharide can induce apoptosis of human K562 cell in vitro. Method：Cells （5 × 10^4/ml） were harvested at mid-exponential phase and then were planted in 96 well plates for 100 μ1 each. Cells were treated for 48 hours with different concentration of Fangfeng Polysaccharide. Growth inhibition rates which induced by fangfeng polysaccharide were measured with MTT method. Apoptosis were detected through following ways., the changes of cell morphology were observed under fluorescence microscope with Hoechst33258 staining, agarose gel electrophoresis was used to detect the DNA fragmentation and flow cytometry with Annexin V and propidium iodide double staining was used to detected apoptosis of K562. Result： Fangfeng Polysaccharide can inhibit proliferation of K562 cells. K562 cells treated with Fangfeng Polysaccharide presented classical apoptotic morphology changes such as cell contraction, and apoptotic bodies under fluorescence microscope, and it showed DNA fragmernt through agarose gel electrophoresis. In addition, the apoptosis percentage which were detected by flow cytometic analysis increase as the fangfeng polysaccharide concentration gose up. Conclusion： Fangfeng Polysaccharide can inhibit proliferation and induce cell apoptosis in K562 in vitro.
出处
《临床血液学杂志》
CAS
2008年第3期260-263,共4页
Journal of Clinical Hematology
