摘要
建立一种快速、稳定、灵敏并可反映细菌活性的细菌计数方法。以乳酸菌中的保加利亚乳杆菌和嗜热链球菌为研究对象,探讨MTT法用于细菌计数的可行性及测量细菌数量的范围、反应时间、MTT的用量、是否加入溶解剂等实验条件。结果:保加利亚乳杆菌在(1.0×105～2.18×107)cfu/mL内测出的OD570值与细菌浓度呈良好的正相关,反应时间1.5h,MTT添加量20μL,测量前用DMSO溶解;嗜热链球菌在(2.0×105～5.12×107)cfu/mL范围内测出的OD570值与细菌浓度呈良好的正相关,反应时间2.0h,MTT添加量20μL,可不添加溶解剂直接测量。MTT比色分析法可用于检测乳酸菌活菌数量。
To establish a rapid, steady and sensitive bacteria-counting method that could reflect the bacteria＇s activity. Lactabacillus delbrueckii ssp. bulgaricus and St reptococcus thermophilus were employed to discuss the feasibility of application of MTT method to bacteria-counting and determine the conditions of the assay, including linear range, reaction time, MTT dosage and whether solvent is added etc.. Results： The optical density at 570 nm is positively related to the concentration of L,delbrueckii ssp. bulgaricus when the number of the bacteria is in the range of （1.0× 10^5-2.18× 10^7） cfu/mg, and reacting time is 1.5 h, MTT dosage is 20 μL, and DMSO is used as solvent before assay; For St.thermophilus, the optical density at 570 nm is positively related to the concentration of the bacteria when the number of the bacteria is in the range of （2.0× 10^5-5.12× 10^7） cfu/mL, and reacting time is 2.0 h, MTT dosage is 20 μL and solvent is not added.. Conclusion： MTT colorimetry could be used to measure the viability of lactic acid bacteria.
出处
《食品工业》
北大核心
2008年第3期62-65,共4页
The Food Industry
基金
国家科技基础条件平台项目(2005DKA21204-08)资助
关键词
MTT
保加利亚乳杆菌
嗜热链球菌
活菌计数
MTT colorimetry
lactobacillus bulgaricus
Streptococcus thermophilus
live germ counting
