摘要
目的:研究从河豚鱼内脏中提取河豚毒素(TTX)的最佳提取条件。用超声提取法与传统的水浴法和回流法进行比较。方法:采用超声提取法对河豚毒素的提取方法进行研究,设计L9(34)正交表,采用紫外分光光度法检测,以提出的TTX含量为评价指标,优选TTX的提取条件。结果:超声时间、乙酸用量、提取次数对TTX的提取量均有影响,其中提取时间有显著影响,最佳提取工艺为用河豚内脏的3倍量的0.1%的乙酸,超声提取3次,每次40min。超声提取法优于水浴法和回流法。
Objective： To choose the optimal method of extracting tetrodotoxin（TTX） from puffer -fish intemal organs. We have compared the result of ultrasonic extraction method with of the method of immersion or backstreaming. Methods： ultrasonic extraction was used to the extraction process of tetrodotoxin ,design L9 （3^4） orthogonal chart to optimize the extraction of TTX, and the detection of tetrodotoxin was determinedby ultraviolet spectrophotometry with content of tetrodotoxin as markers. Results ： ultrasonic time, acetic consumption, extraction times have effect on the content of extracting tetrodotoxin, And the influence of ultrasonic time was significant. The optimal extraction process was 0.1% acetic acid ,3times the amount of material, ultrasonic extraction for 3 times ,40 minutes each time. The ultrasonic extraction method was markedly superior to the method of immersion and backstreaming.
出处
《中华中医药学刊》
CAS
2008年第6期1274-1275,共2页
Chinese Archives of Traditional Chinese Medicine
基金
锦州市科技局资助项目(2005)
关键词
河豚毒素
正交试验
超声提取
紫外分光光度法
tetrodotoxin
orthogonal design
ultrasonic extraction
ultraviolet spectrophotometry
