摘要
采用体外试验研究了3株从斜带石斑鱼仔鱼体内分离的乳酸菌其产酸能力及抑菌特性。结果表明:菌株EA-1和Y1B-3产乳酸的速度和pH值下降速度较快,培养后期乳酸含量显著高于Y4-2(P<0.05)。3株乳酸菌对致病性哈维氏弧菌和溶藻弧菌均有不同程度的抑制作用,但乳酸对三株乳酸菌的抑菌活性没有影响,提示3株乳酸菌均能产生乳酸以外的抑菌物质。三株乳酸菌的抑菌物质具有一定的热稳定性,经60℃和80℃热处理后保留70%以上的抑菌活性,但经100℃高温处理失去抑菌活性。3株乳酸菌产生的抑菌物质对胰蛋白酶敏感,在偏中性条件下抑菌活性较高。
Three strains of lactic acid bacteria, Y4 - 2, EA - 1 and Y1B - 3, were isolated from orange- spotted grouper（ Epinephelus coioides） larvae. Lactic acid production and antagonistic property of three strains were studied in vitro. Results showed that EA- 1 and Y1B- 3 exhibited more rapid production of lactic acid and the amount of lactic acid were higher significantly than that of Y4 -2 in the late fermentation period （P 〈 0.05）. Consequently, pH in the culture of EA - 1 and Y1B - 3 decreased faster than that of Y4 - 2. Culture supernatants of the three strains showed different degrees of antagonistic activity on the pathogenic Vibrio harveyi and Vibrio alginolyticus. However, lactic acid had no effect on the inhibition action of the three strains, suggesting that the bacteria produced other inhibitory substances apart from lactic acid. The inhibition effects of cultures supernatant were above 70% after 15 rain of heat treatment at 60 ℃ or 80 ℃, but lost their inhibition effects after 100 ℃ treatment. The inhibition substances of three strains were susceptive to trypsin and showed higher antagonistic activity at near neutral condition.
出处
《上海水产大学学报》
CSCD
北大核心
2008年第3期344-349,共6页
Journal of Shanghai Fisheries University
基金
国家自然科学基金(30600461)
集美大学科研基金(C60616)
关键词
乳酸菌
抑菌特性
乳酸
胰蛋白酶
lactic acid bacteria
antagonistic property
lactic acid
trypsin
