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HPLC法测定肾移植患者血浆中霉酚酸的浓度 被引量:8

Determination of Mycophenolic Acid in Kidney Transplantation Patients by HPLC
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摘要 目的:建立以高效液相色谱法测定霉酚酸血药浓度的方法,并研究其在中国肾移植患者体内的多剂量药动学特征。方法:血浆采用乙腈蛋白沉淀后进样。其中,色谱柱为Diamonsil C18,流动相为乙腈-10mmol·L^-1KH2PO4(5:6),流速为1.1mL·min^-1,检测波长为254nm,柱温为40℃。此方法应用于测定12例肾移植患者的多剂量药动学。结果:色谱中霉酚酸与内标分离良好,血浆中的内源性杂质无干扰。霉酚酸检测浓度在0.38~59.00μg·mL^-1范围内线性关系良好,最低检测限为0.38μg·mL^-1;回收率为89.32%~97.63%,日内、日间RSD均〈8.00%。药动学结果显示患者服用霉酚酸酯后,霉酚酸的药动学个体差异较大,与文献报道基本一致。结论:本方法结果准确,操作简便,适合于霉酚酸的药动学研究。 OBJECTIVE: To determine the plasma mycophenolic acid concentration by HPLC, and study the multidoses pharmacokinetics character of mycophenolic acid in Chinese kidney transplantation patients. METHODS: The samples were precipitated with acetonitrile before injection. Diamonsil C18 column was used. The mobile phase consisted of acetonitrile- 10 mmol · L^ -1KH2PO4 (5 : 6) at a flow rate of 1.1 mL · min^-1. The detection wavelength was 254 nm and the column temperature was 40 ℃. This method was used to determine the multidoses pharmacokinetics in 12 kidney transplantation patients. RESULTS. MPA was well - separated from internal standard in chromatography, and endogenous foreign substance in plasma had no interference on the determination. The liner range for MPA was 0.38-59.00 μg·mL^-1, and the lowest detectable concentration of MPA was 0.38 μg·mL^-1. The recovery rate stood at 89.32%- 97.63% ; Both intra -day and inter- day RSD were less than 8.00%. Significant individual difference was noted among the patients treated with MMF in pharmacokinetic results, which was in line with the literature. CONCLUSION: This method is accurate and simple and applicable for the pharmacokinetics study of mycophenolic acid.
出处 《中国药房》 CAS CSCD 北大核心 2008年第17期1306-1308,共3页 China Pharmacy
基金 广州市属高校科技项目(61027) 广东省医院药学研究基金资助项目(2008A018)
关键词 霉酚酸 高效液相色谱法 肾移植患者 血药浓度 多剂量 药动学 Mycophenolic acid HPLC Kidney transplantation patients Plasma drug concentration Multidoses Pharmacokinetics
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