摘要
目的了解来源于不同临床标本的肺炎克雷伯菌的耐药性、携带第1类耐药整合子的特征及基因盒的种类．方法使用常规方法分离肺炎克雷伯菌；运用法国梅里埃VITEK细菌分析系统进行鉴定；应用纸片扩散法对15种抗生素进行耐药性监测和分析；应用PCR法扩增第1类整合子；对PCR产物纯化、测序，并对结果进行分析．结果来源于不同临床标本的64株肺炎克雷伯菌100％耐药，32株（50％）表现多重耐药，9株耐4种抗生素，耐药谱为氨苄西林．链霉素．复方新诺明-四环素；20株（31．2％）为第1类整合子阳性株，其中17株整合子大小为1．0kb，2株为1．6kb，1株含有1．0kb和1．6kb两个整合子；PCR产物测序显示1．0kb整合子携带pse-1-aadA1基因盒，1．6kb整合子插入了pse-1-aadA2，pse-1-aacA1基因盒，双重整合子株插入了pse-1-aadA1和pse-1-aacA1基因盒．结论第1类整合子存在于各种临床标本分离的肺炎克雷伯菌中，且与多重耐药性相关，主要决定着对β内酰胺类和氨基糖苷类抗生素的耐药性．
Objective To understand the resistance and the characteristic of class 1 integron carried by K. pneumoniae from different samples, and elucidate the status of gene cassettes. Methods Routine method was used to isolate K. pneumoniae; identified by VITEK; Antibiotic susceptibility was tested with the K-B diffusion method; Class 1 integrons were detected by PCR; PCR products were sequenced and analyzed. Results 64 isolates from different samples were antibiotic resistance, 32 isolates （50%）were multiple drug-resistant, 9 strains resisted 4 kinds of antibiotics, the antibiogram was Ampicillin-Streptomycin-SulfamethoxazoleTetracycline; 20 isolates（31.2% ） were positive class 1 integrons, 17 of 20 integrons size were 1.0 kb which inserted pse-aadA1 gene cassettes when PCR products sequenced; 2 integrons size were 1.6 kb which carried pse-1-aadA2 and pse-1-aacA1 gene cassettes; 1 strains which carried 2 integrons inserted pse-1-aadA land pse- 1-aacA1 gene cassettes. Conclusion Class 1 integrons existed in K. pneumoniae from all kinds of samples, and related to multiple drug-resistant; and determined the resistance of β-lactamases and aminoglycosides antibiotics.
出处
《北华大学学报（自然科学版）》
CAS
2008年第3期229-232,共4页
Journal of Beihua University（Natural Science）
基金
北华大学重点科研项目(200508)
关键词
肺炎克雷伯菌
整合子
耐药性
K. pneumoniae
Class 1 integron
Resistance
