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血清和唾液抗α-胞衬蛋白抗体检测及其在干燥综合征中的意义 被引量:2

Value of serum and saliva anti-α-fodrin antibodies in patients with Sjogren's syndrome
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摘要 目的 了解抗α-胞衬蛋白(α—fodrin)抗体在干燥综合征(SS)患者血清和唾液中的水平,探讨其在诊断中的意义及与临床指标的关系。方法 以酶联免疫吸附试验(ELISA)定量测定25例原发性干燥综合征(pSS)、14例继发性干燥综合征(sSS)、23例其他结缔组织病(CTD)患者、10名健康人血清及唾液中的抗α—fodrin抗体。结果 pSS、sSS血清及唾液中抗α-fodrin IgA、IgG抗体水平高于其他CTD组及正常对照组,差异有统计学意义(P〈0.01),其余各组间比较阳性率差异无统计学意义(P〉0.05)。抗α—fodrin IgA型和IgG型抗体检测阳性率在pss血清及唾液中分别为60%、44%及43%、29%,在sSS分别为52%、36%及36%、29%,在其他结缔组织病血清IgA型和IgG型抗体分别为22%、17%;其他CTD组唾液及正常对照组皿清及唾液中均无一例抗α—fodrin阳性。抗α—fodrinIgA对SS诊断的敏感性为54%,特异性为85%;39例SS患者抗α—fodrin IgA和IgG抗体敏感性低于抗SSA抗体而高于抗SSB抗体,但差异无统计学意义(P〉0.05)。抗α—fodrin抗体的出现与口干、腮腺肿大有关(P〈0.05);与眼干、肺间质病变、肾小管酸中毒、肝损害发生率无关(P〉0.05)。结论 抗α—fodrin抗体在血清和唾液的检测对SS诊断有一定意义,可作为SS诊断的筛选指标。 Objective To evaluate the value of IgA and IgG antibodies against α-fodrin in both serum and saliva in patients with Sjogren's syndrome (SS). The prevalence and clinical associations of anti-α-fodrin antibodies in SS is also assessed. Methods Samples from 39 patients with SS (25 primary and 14 secondary), 8 patients with systemic lupus erythematosus (SLE), and 15 patients with rheumatoid arthritis (RA) as well as 10 healthy blood donors were collected. Anti-α-fodrin antibodies were measured using ELISA. Results The titer of serum anti-α-fodrin was higher in SS than in other connective tissue diseases group and healthy group (P〈0.01). IgA type anti-α-fodrin antibodies was detected in 60%, 44% of serum and saliva in patients with pSS respectively. IgG antibodies were detected in 43% of sera, and 29% of saliva of patients with pSS. The sensitivity and specificity of serum anti-α-fodrin IgA in SS was 54% and 85%. The level of anti-α-fodrin was positively associated with xerostomia and parotid swelling (P〈0.05), and was negatively associated with xeroma, renal tubule acidosis, lung interstitial disease and hepatic damages (P〉 0.05 ), Conclusion Saliva and serum anti-α-fodrin level may be diagnostic for SS. It may be a useful screening marker.
出处 《中华风湿病学杂志》 CAS CSCD 2008年第6期386-389,共4页 Chinese Journal of Rheumatology
关键词 干燥综合征 胞衬蛋白 血清 唾液 Sjogren's syndrome α-fodrin Serum Saliva
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参考文献15

  • 1Vitali C, Bombardleri S, Jonsson R, et al. Classification criteria for Sjogren's syndrome: a revised version of the European criteria proposed by the American-European Consensus Group. Ann Rheum Dis, 2002, 61: 554-558.
  • 2Haneji N, Nakamura T, Takio K, et al. Identification of alpha-fodrin as a candidate autoantigen in primary Sjogren's syndrome. Science, 1997, 25: 604-607.
  • 3Watanabe T, Tsuehida T, Kanda N, et al. Anti-alpha-fodrin antibodies in Sjogren's syndrome and lupus erythematosus. Arch Derrnatol, 1999, 135: 535-539.
  • 4Witte T, Matthias T, Opperrnann M, et al. Prevalence of antibodies against alpha-fodrin in Sjogren's syndrome: comparison of 2 sets of classification criteria. J Rheumatol, 2003, 30: 2157-2159.
  • 5赵岩,费允云,邓学,张佩莲,甘晓丹,史艳萍,周炜,董怡.抗α-胞衬蛋白抗体在干燥综合征诊断中的意义及与临床表现的相关性[J].中华风湿病学杂志,2005,9(5):261-264. 被引量:18
  • 6Zandbelt MM, Vogelzangs J, van de Putte LB, et al. Anti-alphafodrin antibodies do not add much to the diagnosis of Sjogren's syndrome. Arthritis Res Ther, 2004, 6: 33-38.
  • 7Goeb V, Salle V, Duhaut P, et al. Clinical significance of autoantibodies recognizing Sjogren's syndrome A (SSA), SSB, calpastatin and alpha-fodrin in primary Sjogren's syndrome. Clin Exp Immunol, 2007, 148: 281-287.
  • 8De Seze J, Dubucquoi S, Fauchais AL, et al. Autoantibodies against alpha-fodrin in Sjogren's syndrome with neurological manifestations. J Rheumatol, 2004. 31: 500-503.
  • 9Nordmark G, Rorsman F, Ronnblom L, et al. Autoantibodies to alpha-fodrin in primary Sjogren's syndrome and SLE detected lw an in vitro transcription and translation assay. Clin Exp Rheumatol, 2003, 21: 49-56.
  • 10Willeke P, Gaubitz M, Schone H, et al. Clinical and immunological characteristics of patients with Sjogren's syndrome in relation to alpha-fodrin antibodies. Rheumatology (Oxford), 2007, 46: 479-483.

二级参考文献20

  • 1Haneji N, Nakamura T,Takio K, et al. Identification of alpha-fodrin as a candidate autoantigen in primary Sjogren's syndrome. Science, 1997,276 : 604-607.
  • 2Janicke RU, Ng P, Sprengart ML, et al. Caspase-3 is required for alpha-fodrin cleavage but dispensable for cleavage of other death substrates in apopto.sis. J Biol Chem, 1998,273 : 15540-15545.
  • 3Witte T,Matthias T,Arnett FC,et al. IgA and IgG Autoantibodies against A-Fodrin a.s markers for Sj6gren's syndrome. J Rheumatol, 2000,27 : 2617-2620.
  • 4Harris AS, Crall DE, Morrow JS. Calmodulin regulates fodrin susceptibility to cleavage by calcium-dependent protease. J 13iol Chem, 1989,264 : 17401-17408.
  • 5Haneji N, Nakamura T,Takio K,et al. Identification of alpha-fodrin antilxxtiex in Sjogren's syndrome in children. J Rheumatol, 2001,28: 860.
  • 6Adams MI), Kelley JM, Gocayne JD, et al. Complementary DNA sequencing: expressed sequence tags and human genome project, Science, 1991,252:1651-1656.
  • 7Masataka K, Tetsumh O, Yoko O, et al. Autoantibodiee to the amino-terminal fragment of a-fodrin expressed in glandular epithelial cells in patients with Sjogren' s syndrome. J Immunol, 2001, 167 : 5449-5456.
  • 8McMahon AP,Giebelhaus DH,Champion JE, et al. cDNA cloning, sequencing and chromosome mapping of a non-erythroid spectrin, human alpha-Fodrin. Differentiation, 1987,34:68-78.
  • 9Nakano M, Nogami S, Sato S, et al. Interaction of syntaxin with alpha-fodrin,a major component of the submembranous cytoskeleton. Biochem Biophys Res Commun, 2001,288:468-475.
  • 10Inoue H, Tsubota K, Ono M, et al. Possible involvement of EBVmediated alpha-fodrin cleavage for organ-specific autoantigen in Sjogren's syndrome. J Immunol, 2001,166: 5801-5809.

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