摘要
本试验旨在研究伊普异黄酮(IP)对山羊瘤胃代谢以及对瘤胃液雌二醇和睾酮含量的影响。全文包括2组试验:试验Ⅰ为体外培养试验,瘤胃液来自3只装有永久性瘤胃瘘管的本地成年雌性山羊,以0.6g草粉和0.3g精料为底物,研究不同浓度IP(0、10、50、100和200mg/L)对山羊瘤胃代谢的影响;试验Ⅱ为动物试验,选用4只装有永久性瘤胃瘘管的本地成年山羊,采用自身对照设计,试验期在精料中添加IP(1mg/g精料)。试验Ⅰ结果表明,培养液中IP浓度为100和200mg/L时能显著加强瘤胃代谢,pH水平显著下降,氨态氮(NH3-N)浓度和微生物蛋白(MCP)浓度显著升高;试验Ⅱ结果表明,IP有加强瘤胃氮代谢趋势;显著提高乙酸(A)含量(P<0.01)及其比例,使丙酸(P)比例及丙酸/乙酸(P/A)比值显著下降(P<0.01);瘤胃液中17β-雌二醇和睾酮含量显著上升(P<0.05)。提示,IP可以直接作用于瘤胃微生物,加强瘤胃氮代谢,改变瘤胃糖代谢,改变瘤胃微生物发酵类型;显著提高瘤胃液17β-雌二醇和睾酮的水平。
This experiment was conducted to study the effects of ipriflavone on ruminal metabolism and concentration of 17β- estradiol and testosterone in rumen fluid in goat. This study consisted of two experiments, in vitro and in vivo. Ruminal fluid was incubated in anaerobic media in vitro with straw powder （0.6 g） and concentrates （0.3 g） as the substrate. Ipriflavone was added into the incubation with the final concentrations at 0 , 10, 50, 100 and 200 mg/L, respectively. In vivo, four local adult goats with permanent rumen fistulae were conducted into two periods by self-control design, control period and experiment period. In experiment period, ipriflavone was added into concentrates （1 mg/g concentrates） of diet. In exp. 1, results showed that iptiflavone on 100 and 200 mg/L significantly enhanced ruminal metabolism. Levels of pH were reduced, the concentration of NH3-N and MCP were increased obviously. In exp. 2, results showed that ipriflavone increased ruminal nitrogen metabolism in tendency, raised the concentration of acetate （A） and the percentage of acetate （P〈0.01）, lowed the percentage of propionate （P） （P〈0.05） and the ratio of P ： A significantly （P〈0.01） ; Ipriflavone could significantly increase the concentration of 17β-estradiol and testosterone in ruminal fluid of goat （P〈0.05）. These results may suggest that an interaction between ipriflavone and rumen microorganisms occurred. Ipriflavone could enhance nitrogen metabolism in rumen, affect carbohydrates metabolism, alter the fermentation pattern, and significantly increase the levels of 17β-estradiol and testosterone in ruminal fluid.
出处
《动物营养学报》
CAS
CSCD
北大核心
2008年第3期323-328,共6页
CHINESE JOURNAL OF ANIMAL NUTRITION
