摘要
研究了基因重组中华鲟半胱氨酸蛋白酶抑制剂(recombinantChinesesturgeoncystatin)基因在毕赤酵母中的表达和以鲢为原料制作鱼糜过程中添加与未添加recombinantcystatin情况下肌球蛋白的含量变化趋势。结果表明重组cystatin对木瓜蛋白酶具有较强的抑制活性,曲线方程为y=0.0234x+0.0059。SDS PAGE电泳和分析结果表明添加重组cystatin可使鱼糜肌球蛋白具缓和内源性蛋白酶降解作用,具有明显的保护作用。
The study investigated Chinese sturgeon recombinant cystatin gene expression in yeast Pichia pastoris, the inhibitory effect of product on papain, and the trend of myosin＇s content change in processing of silver carp when cystatin were added or not. The result shows that recombinant cystatin can inhibit papain effectively, and the inhibitor curve is ： y = 0. 0234x ＋ 0. 0059. The result of SDS- PAGE of surimi samples were analyzed by the AlphaeaseFC^TM software and it turned that the status that myosin was hydrolyzed by endogenous proteinases were relieved for 45 minutes heating time when 0. 0215 mg·g^-1 cystatin was added to surimi.
出处
《南方水产》
2005年第4期13-17,共5页
South China Fisheries Science
基金
中国水产科学院研究院基金项目(010802)
广东省自然科学基金(021642)
