摘要
利用ISSR标记技术对江蓠属的2个种细基江蓠繁枝变种(Gracilariatenuistipitata)和龙须菜(Gracilarialemaneiformis)的遗传变异进行了研究。所用的细基江蓠繁枝变种材料分别来自粤东(6株)、粤西(3株)、北部湾(3株)及海南岛(5株),龙须菜来自汕头南澳岛。用6条ISSR引物进行筛选,有5条可扩增出清晰可辩条带。这5条引物共扩增72条带,其中53条(73.61%)表现出多态性。根据Nei等的遗传相似性系数进行分析,结果表明:除海南种群外,同一种群中株间相似性达1.000,无任何变异;海南种群各株间相似性在0.899～1.000之间。4个地理群体的细基江蓠繁枝变种的相似系数在0.7955～0.8764之间,北部湾与粤东的江蓠细基繁枝变种的相似性最高(S为0.8764),北部湾与粤西的细基江蓠繁枝变种相似性最低(S为0.7955)。龙须菜与细基江蓠繁枝变种2种间的遗传距离为0.4630。
ISSR technique was used to investigate the genetic diversity of the 2 Gracilaria algae, including 1 strain G. lemaneiformis from Nanao Island and 17 strains G. tenuistipitata var. liui, which came from west Guangdong waters （3 strains）, east Guangdong water （6 strains）, Beibu Gulf （3 strains） and Hainai Island （5 strains） . Of the 6 ISSR primers screened, 5 primers could produce clear bands. A total of 72 bands were amplified and 57 （79. 17% ） revealed polymorphism. The data were used to generate Nei＇s similarity coefficient （S）. The results showed： besides the population of Hainan, there wasn＇t any genetic diversity in individuals interpopulation because their genetic similarity were all 1. 000, the genetic similarity of population of Hainan was among 0. 899 - 1. 000. The genetic similarity were 0. 7955 - 0. 8764 among the four geographical populations of G. tenuistipitata. The maximum similarity occurred between Beibu Gulf and East Guangdong waters （S = 0. 8764 ）. The genetic distance between G. lemaneiformis and G. tenuistipitata was 0. 4630.
出处
《南方水产》
2005年第5期56-59,共4页
South China Fisheries Science
基金
国家"863"高技术研究发展计划项目(2003AA627030)
关键词
细基江蓠繁枝变种
龙须菜
ISSR
遗传变异
Gracilaria tenuistipitata var. liui
G. lemaneiformis
ISSR
genetic diversity
