摘要
目的探讨出生缺陷新生儿中低体质量和肺炎新生儿人巨细胞病毒(HCMV)、单纯疱疹病毒Ⅰ型(HSV-Ⅰ)、HSV-Ⅱ、弓形虫(TOX)、乙肝病毒(HBV)5种病原微生物感染情况,分析巢式聚合酶链反应法检测新生儿病原体感染的临床应用价值。方法选取低体质量新生儿46例和肺炎新生儿66例,每例抽取外周静脉血1mL。经曲酚-氯仿-异戊醇-蛋白消化法,提取外周血血清中的DNA,通过二对引物,一对扩增较大DNA片段的外引物和一对在扩增产物中再扩增小片段的内引物,采用巢式PCR法,分别针对HCMV、HSV-Ⅰ、HSV-Ⅱ、TOX、HBV病毒DNA高度保守区设计引物并扩增其病毒DNA,检测这5种病毒DNA在低体质量和肺炎新生儿中感染阳性率,筛查这些病毒在出生缺陷新生儿中的感染状况。采用SPSS10.0软件分析5种病原体感染率之间的关系。结果低出生体质量新生儿46例中,5种病原体检出率:HCMV为91.3%、HSV-Ⅰ为8.7%、HSV-Ⅱ为15.2%、TOX为8.7%、HBV为15.2%;肺炎新生儿66例中,5种病原体检出率:HCMV为83.3%、HSV-Ⅰ为6.1%、HSV-Ⅱ为16.7%、TOX为6.1%、HBV为7.6%。HCMV感染率在低体质量儿和肺炎患儿中均高于其他4种病原体,且5种病原体感染率有统计学意义(Pa=0)。结论对低体质量和肺炎新生儿病原体感染进行筛查十分必要,且对新生儿感染性疾病的早期诊断和预防有益。
Objective To explore the association between the infection of human cytomegalovirus（ HCMV）, herpes simplex virus type Ⅰ （HSV- Ⅰ ）, HSV-Ⅱ , toxoplasma（TOX） and serum hepatitis B virus （HBV）these 5 pathogens and low body weight and pneumonia, and explore the clinical value of nested polymerase chain reaction （PCR） examining newborns infected with pathogens. Methods Forty - six new- born infants with low weight and 66 newborn infants with pneumonia were selected. And 1 mL pripheral blood of every newborn infant was drawn. Classic phenol - chloroform - isoamyl alcohol - protease digested, after neonatal serum extraction of DNA in peripheral blood through 2 pairs of pri-mers ,the outer primer amplified larger DNA fragments and the irmer primer amplified small fragments,in the amplified products. HCMV,HSV-Ⅰ,HSV-Ⅱ ,TOX and HBV of the viral DNA in highly conservative district to design primer respectively and amplify its viral DNA, nested PCR was used to detect of these pathogens DNA in infants of low body weight and pneumonia,and to detect positive rate of infection. Screening for birth defects in infants in these virus infection. SPSS 10.0 software was used to analyze the relationship between infection of 5 pathogens. Results The infective rate of HCMV in 46 infants with low body quality was 91.3% ,the infective rate of HSV-Ⅰ was 8.7% ,the infective rate of HSV- Ⅱ was 15.2% ,the infective rate of TOX was 8.7% ,and the infective rate of HBV was 15.2%. Among 66 infants with pneumonia,the infective rate of HCMV was 83.3% ,the infective rate of HSV- Ⅰ was 6.1% ,the infective rate of HSV-Ⅱ was 16.7% ,the infective rate of TOX was 6.1%, and the infective rate of HBV was 7.6%. The infective rate of HCMV was higher than that of other 4 pathogens,these infection rates were different statistically in these 5 kinds of pathogens （P = 0）. Conclusions Five kinds of pathogens both low pathosens screening is necessary newborns infants with low body weight and pneumonia, and for the early diagnosis and prevention of these pathogens.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2008年第10期739-741,共3页
Journal of Applied Clinical Pediatrics
基金
国家自然科学基金项目资助(30571604)
关键词
婴儿
新生
低出生体质量
人巨细胞病毒
单纯疱疹病毒Ⅰ型
单纯疱疹病毒Ⅱ型
弓形虫
乙肝病毒
巢式聚合酶链反应
infant, newborn
low birth body weight
human cytomegalovirn
herpes simplex B virus type Ⅰ
herpes simplex virus type Ⅱ
toxoplasma
hepatitis B virus
nested - polymerase chain reaction
