摘要
目的:观察急性肝损伤、暴发性肝衰竭时肝再生的变化及血浆内毒素变化对暴发性肝衰竭肝再生的影响。方法:硫代乙酰胺复制动物模型,鲎试剂法测定血浆内毒素含量,流式细胞仪及增殖细胞核抗原免疫组化测定细胞增殖情况。结果:暴发性肝衰竭增殖细胞核抗原标志指数及肝细胞增殖指数较急性肝损伤明显增高(P 值<0.05);暴发性肝衰竭早期血浆内毒素与细胞增殖指数呈正相关(r=0.8745 P 值<0.01),但暴发性肝衰竭晚期血浆内毒素与细胞增殖指数呈负相关(r=-0.7776 P 值<0.01),而此时血浆内毒素与血浆转氨酶(AST)呈正相关(r=0.7391 P 值<0.05)。结论:暴发性肝衰竭动物有较强的再生能力,肠源性内毒素血症与暴发性肝衰竭肝损伤及肝再生密切相关。
Objective:To observe liver regeneration in fulminant hepatic failure(FHF)and in acute liver injury induced by thioacetamide in rats,and effect of plasma endotoxin changes on the liver regeneration during the development of FHF.Methods:Plasma endotxin level was analysed by colorimetric limulus test using chromogenic substrate,and liver regeneration by flow cytemetric analysis and proliferating cell nu- clear antigen(PCNA)immunohistochemical staining.Results:Both PCNA labeling index and hei)atocyte proliferating index were significantly higher in FHF than that in acute liver injury(P<0.05).There was position correlation between the endotoxin level and hepatocyte proliferating index in the early stage of FHF(r=0.8745 P<0.01)、but、 negative correlation in the late stage of FHF(r=-0.7776 P<0.01).However, there was positive correlation between the endotoxin level and the value of AST in the late stage(r=0.7391 P<0.05).Conclusion:There was a potent ability to regeneration in FHF liver injury and hepatic regenera- tion after liver injury were closely correlated to intestinal endoxemia.
出处
《中华肝脏病杂志》
CAS
CSCD
1997年第4期222-224,共3页
Chinese Journal of Hepatology
关键词
暴发性肝衰竭
肝再生
内毒素血症
实验
Fulminant hepatic Failure
Liver regeneration
Intestinal endotoxemia
