摘要
目的：观察粉防己碱（ｔｅｔｒａｎｄｒｉｎｅ，Ｔｅｔ）对缺血顿抑心肌的保护作用并分析与心肌ＡＴＰ酶活力的关系。方法：离体大鼠工作心脏，全心缺血３０ｍｉｎ再灌４０ｍｉｎ，无机磷显色法分析ＡＴＰ酶活力。结果：顿抑心肌收缩舒张功能均明显降低，再灌期末ＬＶＳＰ×ＨＲ仅恢复５２％±８％，ＬＶＥＤＰ抬高２９８％±６４％，ＣＯ仅恢复４０％±８％，心肌细胞膜和线粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活力均下降，Ｔｅｔ（３０ｍｇ·ｋｇ－１·ｄ－１，ｉｐ，３ｄ，１０μｍｏｌ·Ｌ－１灌流缺血全程）可使ＬＶＳＰ×ＨＲ恢复８５％±１２％，ＬＶＥＤＰ仅抬高１６６％±４４％，ＣＯ恢复７５％±１１％，心肌细胞膜及线粒体Ｎａ＋，Ｋ＋－ＡＴＰ酶和Ｃａ２＋，Ｍｇ２＋－ＡＴＰ酶活力增高。结论：Ｔｅｔ对心肌缺血后顿抑损伤有一定保护作用，这一作用与维护顿抑期心肌细胞ＡＴＰ酶活力有关。
AIM: To study the protective effect of tetrandrine (Tet) on postischemic myocardial stunning and whether it is responsible for ATPase activity. METHODS: Isolated working rat hearts, global ischemia for 30 min followed by reperfusing for 40 min. RESULTS: Both systolic and diastolic function of stunned myocardium were significantly decreased. The percent recovery of LVSP×HR and CO was 52%±8% and 40%±8%, respectively; LVEDP and T were elevated; while both Na+,K+ATPase activity and Ca2+,Mg2+ATPase activity of myocardial membrane and mitochondria were depressed. Tetrandrine (30 mg·kg-1·d-1, ip, 3 d, and 10 μmol·L-1 for isolated heart perfusion) was able to enhance the recovery of LVSP×HR and CO to 89%±12% and 80%±14%, respectively, and reduce LVEDP from 298%±64% to 165%±55%, with an improvement in myocardial membrane and mitochondria Na+,K+ATPase and Ca2+,Mg2+ATPase activity. CONCLUTION: This study suggested that tetrandrine can protect cardiac function from ischemia reperfusion stunning injury, which may be related to preserving ATPase activity in ischemic myocardium.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1997年第6期548-551,共4页
Chinese Pharmacological Bulletin
基金
江苏省教委资助
关键词
心肌缺血
再灌注损伤
顿抑
ATP酶
粉防己碱
ischemia reperfusion injury
myocardial stunning
tetrandrine
ATPase activity
