摘要
运用AFLP技术,对广东鲮鱼原种场保存的鲮原种2个子群体(子群体h、q)各29个个体进行遗传多样性分析。25对AFLP引物组合中的6对引物扩增共产生173条条带,其中多态位点数为72,多态位点比例为41.5%。在6对引物产生的173条扩增条带中,有一条扩增条带在子群体h中的出现频率远远高于其在子群体q中的出现频率(72.4%>20.6%),编号为E2M4-1。遗传多样性分析结果表明,子群体h的遗传多样性指数高于子群体q(0.1367>0.0998)。该研究为筛选鲮原种群体2个子群体间的特异性分子标记做了初步尝试。
Amplified fragment length polymorphism （AFLP） technique was applied to assess the genetic variations of the original pop- ulation collected from Original Species Field of Cirrhinus molitorella in Guangdong Province. The studied samples contained sub-stock h and sub-stock q, 29 individuals respectively. Amplifications with 6 pairs of primers gave 173 reproducible and stable fragments, of which 72 were polymorphic. There was one band whose frenquency was much higher in sub-stock h than that was in sub-stock q （72.4% 〉20. 6% ） . We named it as E2M4-1. Based on AFLP data, we found that the genetic diversity of sub-stock h was higher than sub-stock q （0. 1367 〉0. 0998）. This study is a preliminary attempt for special molecular marker selecting of the two wild substocks .
出处
《南方水产》
2007年第3期57-60,共4页
South China Fisheries Science
基金
广东省自然科学基金(033)
关键词
鲮
扩增片段长度多态性
遗传多样性
mud carp
Cirrhinus molitoreUa
amplified fragment length polymorphism （AFLP）
genetic diversity
