摘要
目的研制低密度脂蛋白胆固醇(LDL-C)表面活性剂直接消除法测定试剂。方法低密度脂蛋白(LDL)与试剂Ⅰ中的表面活性剂Ⅰ形成稳定复合物,而非LDL中胆固醇被胆固醇酶、过氧化物酶消除,反应5 min后,加入试剂Ⅰ,与表面活性剂Ⅱ和色原反应,LDL释放胆固醇并显色,LDL的浓度与呈色的深浅成正比。结果LDL-C检测试剂:在方法性能测定中,在2 min的测定时间内,LDL-C浓度5.0 mmol/L-27.0 mmol/L范围内,线性关系良好(r=0.9912),特异性良好,平均回收率为99.52%,LDL-C低、中、高值血清标本的批内和批间变异系数(CV)分别为2.6%-3.3%和3.5%-4.2%,乳糜微粒、胆红素和血红蛋白等干扰不明显;方法对比实验表明,其与PVS沉淀法、Friedewald公式法及其日本第一化学试剂相关良好,r值分别为0.967,0.9913,0.9779。结论LDL-C检测试剂各项指标符合临床要求。
Objective To study direct eliminating assay reagents for measuring low-density lipoprotein cholesterol. Methods Low-density lipoproteinwas combined with detergent Ⅰ in regeant Ⅰ . Cholesterol esterase, cholesterol oxides, and peroxidase eliminated the non-combined cholesterol of non-LDL esters in five minutes. Subsequently the combined LDL was released by the detergent Ⅱ ; the enzyme reaction with LDL-C in the presence of the coupler produced color in proportional to the amotmt of LDL cholesterol in the sample. Results The directly detemfined LDL- C values firstly, in the assay method of capability, the assay was linear up to between 5.0 mmol/L and 27.0 mmol/L of LDL-C（ r = 0.9912） in two minutes, secondly, the specificity was better and the mean recovery rate was 99.52%, ttirdly, in the precision assay the inter and intra CV at low, middle, high concentration specimens were 2.6 % - 3.3 % and 3.5% -4.2% respectively, lastly, interference with Chylomicron, bilimbin and hemoglobin were neglected. On the other hand, in the contrast methods, the directly detemfined LDL-C values were closely correlated with those obtained by precipitation with PVS, F formula and Japanese reagent, and the r value of them were separately 0.967,0.9913,0.9779. Conclusion The capability of the LDL-C assay meeted the clinical demand.
出处
《中国实验诊断学》
2008年第6期699-702,共4页
Chinese Journal of Laboratory Diagnosis
关键词
表面活性剂
匀相法
低密度脂蛋白胆固醇
detergen
Homogeneous method
low-density lipoprotein cholesterol
