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Interactions of the HSV-1 UL25 Capsid Protein with Cellular Microtubule-associated Protein

Interactions of the HSV-1 UL25 Capsid Protein with Cellular Microtubule-associated Protein
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摘要 An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the UL25 protein demonstrated its co-localization with cellular microtubule-associated protein in the plasma membrane. Further investigations with deletion mutants suggest that UL25 is likely to have a function in the nucleus. An interaction between the HSV-1 UL25 capsid protein and cellular microtubule-associated protein was found using a yeast two-hybrid screen and β-D-galactosidase activity assays. Immunofluorescence microscopy of the UL25 protein demonstrated its co-localization with cellular microtubule-associated protein in the plasma membrane. Further investigations with deletion mutants suggest that UL25 is likely to have a function in the nucleus.
作者 Lei GUO Ying ZHANG Yan-chun CHE Wen-juan WU Wei-zhong LI Li-chun WANG Yun LIAO Long-ding LIU Qi-han LI
机构地区 Institute of Medical Biology
出处 《Virologica Sinica》 SCIE CAS CSCD 2008年第3期211-217,共7页 中国病毒学（英文版）
基金 National Natural Science Funds(30570081, 30670094 and 30700028)
关键词 HSV-1 CAPSID UL25 Microtubule-associated protein 衣壳 蛋白质 细胞 萤光免疫检验法 检验方法
分类号 Q51 [生物学—生物化学] Q26 [生物学—细胞生物学]
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参考文献15

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Virologica Sinica
2008年 第3期

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