摘要
目的:探讨小站台水环境应激不同时间对小鼠行为、免疫器官的变化及免疫功能与性激素水平的影响,为进一步研究心理应激条件下动物行为和机体免疫功能的变化提供实验依据。方法:利用小站台水环境诱发的应激模型,观察站台12h、24h、48h和对照组在站台前后动物一般行为、胸腺和脾脏重量与指数的变化以及性激素水平的变化状况。结果:应激使小鼠的一般情况发生改变,表现为体重减轻,易受惊吓,高度紧张,攻击性增强,呼吸急促,对外界环境干扰敏感度低,修饰行为减少,体能水平逐渐降低,运动能力逐渐下降;应激24h、48h组胸腺重量和指数改变与对照组相比有显著性差异(P<0.05),应激12h、24h、48h组小鼠脾脏指数改变显著,与对照组相比有显著性差异(P<0.05);应激12h、24h、48h组小鼠血清皮质醇(Cortisol)、醛固酮(ALD)含量均有极显著变化,与对照组相比有极显著性差异(P<0.01);应激24h、48h组小鼠血清睾酮(T)、雌二醇(E2)含量与对照组相比有极显著性差异(P<0.01)。结论:心理应激可引起小鼠行为、体重、激素水平和免疫功能的改变,从而引起体质衰弱、病态或疾病。
Objective To study the animal behavior and immuno -function changes under psychostressful conditions through investigating those reactions in mouse models of stress. Methods Mouse models of stress （ n = 60） were prepared with the animals isolated and confined to a 4cm^2 wooden platfrom 20cm above water surface for 12h （n =20）, 24h （ n =20） or 48h （n =20）. The animals were sacrificed after the experiment： thymas and spleen weight were measured in half of the animals and serum cortisol, aldosterone, E2, T levels were measured with RIA in the other half. Twenty animals were kept under hormal enviromenat and served as controis. Results The genelal behavior in the stressful models changes markedly with evidence in favor of anxiety resulting in decreased stamina. The thymas weight and thymic index （ at 24h, 48h） as well as splenic weight and splenic index （ at 12, 24 and 48h） in the models were significantly lower than those in the controls （ P 〈 0.05 ）. The serum cortisol and aldosterone levels in the models at 24 and 48h were significantly higher than those in the controls （ P 〈 0.01 ） while the E2, T, levels were significantly lower （ P also 〈 0. 01 ）. Conclusion Psychostress could induce behavior changes with alteration of immuno - function and hormone levels in the experimental animals （mouse models of stress）, resulting in deterioration of general health condition.
出处
《放射免疫学杂志》
CAS
2008年第3期215-217,共3页
Journal of Radioimmanology
关键词
心理应激
免疫功能
性激素
phychostreas, immuno - function, sex - hormone
