摘要
研究了食品中胭脂虫红色素的高效液相色谱测定方法。含胭脂虫红色素的样品用液-液萃取后通过高效液相色谱进行分析，并通过比较测定的光谱与胭脂虫红的紫外-可见谱图库来进一步确证。采用Welch Materials XB-C18（4．6mm×250mm，5μm）柱，流动相为0．02mol／L乙酸铵溶液（A）和甲醇（B），梯度洗脱，流速为1．0mL／min，检测波长为280nm，外标法测定胭脂虫红的含量。结果表明，胭脂虫红在1．0～50．0mg／L范围内线性关系良好，回收率为88％～99％，检出限为0．041mg／L，色谱峰分离效果好，具有良好的稳定性和重复性。
A sensitive HPLC method for the analysis of carminic acid in food was described. Food samples were extracted by liquid - liquid extraction and chromatographied on a Welch Materials XB - C28 （4- 6 mm ×250 mm, 5 μm） column and detected at 280 nm, with a mobile phase of 0. 02 mol/L ammonium acetate solution -methanol（ gradient elution） at a flow rate of 1.0 mL/min. The linearity was obtained in the range of 1.0 - 50. 0 mg/L （ r = 0. 999 9 ）. The determination limit was 0. 041 mg/L. The average recoveries of samples was in the range of 88% - 99% . The analytical method for carminic acid in food was proved to be simple, sensitive and reliable.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第6期648-650,共3页
Journal of Instrumental Analysis
关键词
高效液相色谱法
二极管阵列检测器
胭脂虫红
糖类食品
肉制品
high performance liquid chromatography
diode array detector
carminic acid
carbo- hydrate foods
meat products
