摘要
目的观察小脑顶核电刺激预处理对大脑中动脉闭塞（MCAO）大鼠脑组织基质金属蛋白酶（MMP-9）表达的影响。方法选择雄性Wistar大鼠，随机分为对照组、小脑顶核刺激组（FNS组）及假FNS组。采用线栓法建立大鼠MCAO模型，观察时间点为MCAO后第3，6，12，24，及72小时。对照组行假手术，并不栓塞大脑中动脉。FNS组大鼠预先电刺激小脑顶核1h，24h后行MCAO。假FNS组仅将针电极置人大鼠小脑顶核，但不予通电。采用干湿重法测定脑含水量，Evan蓝法测定血-脑屏障（BBB）通透性，免疫印迹法测定MMP-9的表达。结果与对照组比较，假FNS组MCAO后各时间点缺血侧脑含水量增加，BBB通透性第6小时后增高，MMP-9表达增强；与假FNS组比较，FNS组大鼠MCAO后各时间点缺血侧脑含水量明显下降，BBB通透性第6小时后明显降低，MMP-9表达明显减弱（P〈0．05）。结论预先电刺激小脑顶核，可抑制缺血区脑组织MMP-9的表达，从而抑制BBB通透性的增高，改善缺血区组织水肿。
Objective To investigate the effect of preconditioning through stimulation of the fastigial nucleus （FNS） on the expression of matrix metalloproteinase-9 after cerebral ischemia. Methods A total of 312 male Wistar rats were divided randomly into three groups： a control group, a sham-FNS group and a FNS group. All the rats in the sham-FNS and FNS groups were subjected to middle cerebral artery occlusion （MCAO） by using a thread to occlude the right middle cerebral artery, while those in the control group were subject only to a similar operative procedure without real MCAO. Twenty-four hours before the operation, all the rats in the FNS group were given 1 hour of preconditioning FNS, while those in the sham-FNS group was given sham-FNS. Individuals were sacrificed at the 6th, 12th, 24th, and 72nd hour after MCAO. The brain water content was measured by the dry-wet weight method. The blood-brain barrier （BBB） permeability was measured using Evan＇s blue. MMP-9 protein expression was measured by Western blotting. Results The water content in the ischemic brain tissue, the BBB permeability and the expression of MMP-9 were all found to be significantly higher in the sham-FNS group than in the control group. Compared with the sham-FNS group, the brain water content and BBB permeability were significantly lower in the FNS group, while MMP-9 protein expression also decreased significantly. Conclusion FNS preconditioning can inhibit MMP-9 expression, thereby relieving BBB permeability and ischemic brain edema.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2008年第5期294-297,共4页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
小脑顶核
脑缺血
血-脑屏障
基质金属蛋白酶-9
Fastigial nucleus
Cerebral ischemia
Blood-brain barrier
Matrix metalloproteinase-9
