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颈交感神经干离断对局灶性脑缺血再灌注后大鼠海马iNOS表达的影响

Effect of transection of cervical sympathetic trunk on the expression of iNOS in Hippocampus of rats during focal cerebral ischemia reperfusion
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摘要 目的采用颈交感干离断(TCST)模拟星状神经节阻滞,观察其对局灶性脑缺血再灌注损伤(CIRI)大鼠脑梗死容积及海马诱导型一氧化氮合酶(iNOS)表达等的影响,并探讨其脑保护作用的机制。方法将大鼠随机分成实验组(A组)、对照组(B组)和假手术组(C组);采用线栓法行大脑中动脉栓塞(MCAO)制作大鼠局灶性CIRI模型,A组于TCST后即行MCAO,2h后再恢复灌注;B组为单纯CIRI组;C组仅完成与A组相似的手术步骤但不造成MCAO、不行TCST;再灌注24h后观察各组大鼠神经行为学评分、脑梗死容积及海马iNOS的表达变化。结果A组大鼠脑梗死容积和神经行为学评分均低于B组(P<0.05);与A组、C组相比,B组大鼠海马iNOS的表达增加(P<0.05),而A组与C组间无显著差异(P>0.05)。结论TCST可通过下调大鼠海马iNOS的表达而对局灶性CIRI发挥脑保护作用。 Objective To use transection of cervical sympathetic trunk (TCST) to simulate stellate ganglion block(SGB), to investigate its effects on cerebral infarct volume, the expressin of induced nitric oxide synthase(iNOS) in Hippocampus of rats during focal cerebral ischemia reperfusion injury(CIRI) ,and analyzing its neuroprotective mechanism. Methods All the animals were randomly divided into three groups: experiment group(group A),control group(group B)and sham-operation group(group C). focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO) with the intraluminal suture method. Group A with MCAO for 2 h followed by reperfusion after TCST;Group B only with CIR;In group C, the similar operation procedure were complete only but on MCAO and no TCST. The neurological deficit scores, the infarct size and the expression of iNOS were detected 24 h after reperfusion. Results The cerebral infarct size and neurological deficit scores of rats in group A were lower than that in group B(P〈0. 05). The expression of iNOS in Hippocampus of rats in group g increased obviously campared to group A and C (P〈0.05) ,but there were no difference between group A and C(P〉0. 05). Conclusions TCST was neuroprotective against focal CIRI throught decreasing the expression of iNOS in Hippcampus of rats.
出处 《卒中与神经疾病》 2008年第3期142-144,147,共4页 Stroke and Nervous Diseases
基金 湖北省教育厅优秀中青年人才项目(No2002B03001)
关键词 颈交感干 脑缺血再灌注 一氧化氮合酶 Cervical sympathetic trunk Cerebral ischemia reperfusion iNOS
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参考文献16

  • 1Iadecola C. Bright and dark sides of nitric oxide in ischemic brain injury. Trends Neurosci, 1997,20(3) : 132-139.
  • 2Iwama H. Can superior cervical ganglionectomy be an experimental model of stellate ganglion block? Masui, 1997, 46 (4) : 565- 567.
  • 3全守波,王清秀,刘菊英,杨光,傅南安,朱涛.星状神经节阻滞对全脑缺血再灌注损伤家兔海马及颞叶皮质热休克蛋白70表达的影响[J].中华麻醉学杂志,2004,24(2):133-137. 被引量:23
  • 4全守波,刘菊英,王清秀,杨光,傅南安.星状神经节阻滞对全脑缺血-再灌注损伤家兔血浆内皮素和降钙素基因相关肽的影响[J].临床麻醉学杂志,2005,21(4):260-262. 被引量:36
  • 5Longa EZ,Weinstein PR,Carlson S, et al. Reversible middle cerebral artery'occlusion without eranieetomy in rats. Stroke, 1989,20 (1):84-91.
  • 6Wright LL. Development of the sex difference in neuron numbers of the superior cervical ganglion:effects of transection of the cervical sympathetic trunk. J Comparative Neurology, 1987,263 (2) : 259-264.
  • 7Bederson JB, Pitts LH,Davis RL, et al. Rat middle cerebral artery occlusion: Evaluation of the model and development of a neurologic examination. Stroke, 1986,17(3) : 472-476.
  • 8熊良志,王清秀,周青山,肖峰.星状神经节阻滞对全脑缺血再灌注后家兔海马CA1区Bax及Bcl-2表达的影响[J].中国临床康复,2006,10(14):105-107. 被引量:5
  • 9Kagawa T, Horie R, Moritake K, et al. Effects of long-term cerebral ischemia caused by bilateral carotid artery ligation onthe acceleration or the development of hypertension in spontaneously hypertensive rats(SHR) or Wistar-kyoto rats(WKY). Clin Exp Hyertens A, 1991,13(5):1043-1049.
  • 10Lerouet D,Beray-Berthat V,Palmier B, et al. Changes in oxidative stress, iNOS activity and neutrophil infiltration in severe transient focal cerebral ischemia in rats. Brain Res, 2002, 958 (1) :166-175.

二级参考文献82

  • 1张庆.颅内血肿血量的CT测量[J].实用放射学杂志,1993,9(8):497-497. 被引量:2
  • 2王吉村,莫简,孙长凯,王多宁,孙淑芬,胡家露,张忠兵,唐卫忠.组织及组织液中—氧化氮的荧光分光光度法检测[J].第四军医大学学报,1995,16(6):463-464. 被引量:31
  • 3孙青芳 赵卫国 卞留贯 等.脑缺血对cNOS、iNOS基因表达的影响[J].中华神经科杂志,1999,32:119-119.
  • 4[1]Longa EL. Reversible middle cerebral artery occlusion without cranietomy in rats. Stroke, 1998, 20:84
  • 5[2]Samdani AF, Dawson TM. Dawson VL, et al. Nitric oxide synthase in models of focal ischemia. Stroke, 1997, 28:1283
  • 6[3]Nelson CW, Wei EP, Povlishak JT, et al. Oxygen radicals in cerebral ischemia. Am J Physiol, 1992, 263:1356
  • 7[4]Iadecola C, Xu F, Zhang FY, et al. Marked induction of calciumdependent nitric oxide synthase activity after cerebral ischemia. J Cereb Blood Flow Metab, 1995, 15:52
  • 8[5]Tsuji M, Higuchi Y, Shiraishi K, et al. Protective effect of aminoguanidine on hypoxic-ischemic brain damage and temporal profile of brain nitric oxide in neonatal rat. Pediatr Res, 2000, 47(1): 79
  • 9[6]Cash D, Beech JS, Rayne RC, et al. Neuroprotective effect of aminoguanidine on transient focal ischaemia in the rat brain. Brain Res, 2001, 905(1-2): 91
  • 10[7]Barone FC, Schmidt DB, Hillegass LM. Reperfusion increases neutrophils and leukotriene 134 recrptor binding in rat focal ischemia.Stroke, 1992, 20(9): 1337

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