摘要
以磷效率差异显著的垦鉴27(磷高效型)和丰收25(磷低效型)2个品种为供试材料,采用盆栽控制磷供应水平方法研究了不同基因型大豆各生育时期的细胞膜透性、SOD、POD、CAT活性以及MDA积累状况。结果表明:2种基因型大豆在磷供应充足的情况下,保护酶活性差异不显著;低磷胁迫下,丰收25细胞膜透性大幅度升高,相对电导率升高41.01%,MDA含量增加23.43%,保护酶活性明显下降,SOD活性降低12.52%,POD活性降低24.16%,CAT活性降低31.75%;垦鉴27细胞膜透性稍有增加,相对电导率升高19.39%,MDA含量增加14.11%,保护酶活性略有降低,SOD活性降低4.98%,POD活性降低13.21%,CAT活性降低28.81%。由此说明,垦鉴27可以通过对磷素高效利用,提高保护酶(SOD,CAT和POD)活性降低O.2-的产生速率,从而降低细胞膜透性,而丰收25这种自我保护能力较差。
Plant cuhivars with high phosphorus efficiency adapt to P stress environment easily. Previous studies have focused on the mechanism of rice and wheat＇ s adaptation to low P stress. But effect of low phosphorus stress on activities of cell defense enzymes of different P efficient soybean is less documented. Therefore,pot experiments were conducted in 2006 year, with P high efficient soybean Kenjian 27 and P low efficient soybean Fengshou 25 as materials, to determine effect of low phosphorus stress on activities of cell defense enzymes of different P efficient soybean. The genotypic differences in P uptake efficiency of two soybean varieties were studied under controlled phosphorus （P） nutrition status, endosmosis permeability, superoxide dismutase （ SOD）, peroxide enzyme （POD） activity, catalase enzyme （CAT） activity, and malondiaidehyde （MDA） content of different genotypic soybean growth period were investigated in nutrient solution culture. The difference of activities of cell defense enzymes was not significant under sufficient phosphorus supply. Under low phosphorus stress, endosmosis permeability of Fengshou 25 sharply increased, with conductivity and MDA content increased 41.01% and 23.43% ,and activities of cell defense enzymes reduced obviously, with activities of SOD, POD and CAT decreased 12. 52 % ,24.16 % , and 31.75 %, respectively ; while for Kenjian 27, endosmosis permeability increased slightly and activities of cell defense enzymes were slightly reduced either. Results suggest that phosphorus high efficient soybean Kenjian 27 could make full use of phosphorus, improve activity of cell defense enzymes, depress bringing speed of O2^·- , reduce endosmosis permeability.
出处
《大豆科学》
CAS
CSCD
北大核心
2008年第3期437-441,共5页
Soybean Science
基金
"十一五"国家科技支撑计划资助项目(2006BAD21B01-6)
黑龙江省"十一五"科技攻关资助项目(GA06B101-2-5
GA06B101-1-2)
黑龙江省自然科学基金资助项目(C2007-13)
大豆产业技术体系资助项目
农业部寒地作物生理生态重点开放实验室基金项目
关键词
大豆
基因型
保护酶活性
低磷胁迫
Soybean
Genotype
Activities of cell defense enzyme
Low phosphorus stress
