摘要
背景:胰岛素抵抗是代谢综合征、糖尿病和动脉粥样硬化的共同病理基础。目的:比较相同体质量不同饮食诱导及不同体质量相同饮食诱导建立胰岛素抵抗大鼠模型的可行性。设计、时间及地点:随机对照动物实验,于2006-10/2007-09在泰山医学院生命科学研究所完成。材料:SPF级1个月龄雄性SD大鼠20只;胰岛素溶液为江苏万邦生化医药股份有限公司产品,按照4mU/(kg·min)的滴速标准用前临时配制。方法:按体质量分组4组:体质量在(140±10)g范围的为普通饲料组、高盐高脂饲料组、高糖高脂饲料组,体质量在(180±10)g范围为高糖高脂饲料组,每组5只。主要观察指标:用高脂饮食诱导建立胰岛素抵抗模型;用正常葡萄糖-高胰岛素钳夹试验验证是否有胰岛素抵抗;不同体质量、不同饮食配方与胰岛素抵抗模型的关系。结果:轻体质量和重体质量大鼠在基础血糖、基础血压方面差异无显著性,20只大鼠全部进入结果分析。①高脂喂养4个月后,两组大鼠体质量、肾脏和附睾周围脂肪重量及GIR60-120差异已无显著性意义,血压差异有显著性意义(P<0.05),Ln(FINS)、Ln(SINS)差异有极显著性意义(P<0.01)。②体质量(140±10)g大鼠经高脂喂养4个月后,高脂喂养组与普通饲料组体质量差异无显著性意义。肾脏周围脂肪重量、喂养后血压、Ln(FINS)、Ln(SINS)及GIR60-120差异都有极显著性意义(P<0.01);高脂高糖及高脂高盐喂养大鼠除Ln(SINS)差异有极显著性意义外,其他指标之间差异无显著性意义。结论:基础体质量较低的大鼠易出现胰岛素抵抗,高脂饮食能更易成功诱导低体质量大鼠胰岛素抵抗模型,在高脂喂养中,饲料中的高糖与高盐对胰岛素抵抗生成影响的差别不大。
BACKGROUND： Insulin resistance is a common pathological base of metabolism symptom, diabetic mellitus and atherosclerosis. OBJECTIVE： To verify the feasibility of establishing insulin resistance model in rats by different diets under the same weight versus the same diet under different weights. DESIGN, TIME AND SETTING： The random controlled animal experiment was carried out at the Institute of Life Sciences in Taishan Medical College between October 2006 and September 2007. MATERIALS： Twenty male SD rats, one month old and SPF grade were adopted; insulin solution, the product of Wanbang Biochemical Pharmaceutical Company, was prepared according to the dropping speed of 4 mU/（kg·min）. METHODS： Rats were divided into four groups according to the weight： light weight [（140±10） g] rat groups with standard food, high-fat and high-sugar diet, as well as high-fat and high-salt diet, respectively. In addition, high weight [（180±10） g] rat group was also included. Each group contained 5 rats. MAIN OUTCOME MEASURES： Insulin resistance model was induced by high-fat feeding, and insulin sensitivity was valued by normal glucose-hyperinsulinemic euglycemic clamp. The relation of insulin resistance with different weights and different diets was also investigated. RESULTS： All of 20 rats went into the result analysis, without the significant differences of basic blood glucose and basic blood pressure. After four-month high-fat diet, there was a significant difference in the blood pressure （P 〈 0.05）, but no significant difference in the body weight, peripheral fat weight of kidney and parastata, together with glucose infusion rate （GIR60-120）. Extremely significant differences were found in the Ln （FINS） and Ln （SINS） （P 〈 0.01）. After four-month high-fat diet, high-fat diet groups had greater body weight than normal controls, but there was not significant difference. Compared with normal controls, there were extremely significant differences in the fat weight of the periphery of kidney, feeding blood pressure, Ln （FINS）, Ln （SINS） and GIR60-120. Except Ln （SINS） exhibited extreme significances, there was no significant difference in the other indexes between two high-fat groups. CONCLUSION： The high-fat feeding is prone to induce insulin resistance model in rats, and the light basic weight rats benefit this process. Neither high-sugar nor high-salt of high-fat diet carries obvious influences on the induction of insulin resistance.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第24期4689-4692,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
