摘要
目的:建立一种单蒂阻隔皮瓣模型,用以观察药物对皮瓣存活长度的影响。方法:以鼠背部中线为中轴,以两侧髂嵴连线为蒂,形成一个1.5cm×6cm的单蒂皮瓣,其全长自皮肌层下与深筋膜游离,下垫置医用硅膜,硅膜以不露出切口又起到阻隔真皮血运为宜;以小三角针用3-0丝线先缝合皮瓣两侧切口,然后将药物加入皮瓣与硅膜之间,再缝合皮瓣的头端;术后单笼喂养7天,取材并检测皮瓣存活的长度,根据均数与总体比较的样本量估算公式,进行模型可重复性推算。结果:单蒂阻隔皮瓣模型在展示10%的分辨率时所需的样本量为26只大鼠,而应用10cm×3cm鼠背部皮瓣模型,则需动物为28只,25%检验水平时所需样本量分别为2只及5只,因此,认为阻隔皮瓣动物模型,具有较高的可重复性,符合实验设计的基本原则,适用于该类实验研究。结论:大鼠背部单蒂阻隔皮瓣模型,是进行皮瓣药物学研究较为理想的动物模型。
Objective To design a new flap model using for study on effects of some drugs which would maximize flap survival, and to evaluate its experimental availability.Methods The Wistar rat was used as the experimental animal. On the back of each rat, a skin flap, measuring 1.5 cm × 6 cm, caudally based, was elevated and separated. Wound edges and the flap bed were separated from the flap by a silicone sheet and the drug was put in the areas between flap and sheet. On the seventh day after the flap elevation, historical and physiological measurement to blood flow and skin specimens would be done. The repeatability of the model was calculated. Results Single pedicle separaration flap needed 26 rats as samples when the resolution was 10% in testing, while the back flap model （10 cm × 3 cm ） needed 28 rats. The needed samples were 2 rats and 5 rats respectively when the resolution was 25%. So it was believed that with high repeatability, the model of pediele separation flap met the basic principle of testing design and was suitable for the study of the same kind. Conclusion The model can be used with confidence to study the effects of drugs on the flap survival. [Chinese Medioal Equipment Journal ,2008,29（6）： 17-18]
出处
《医疗卫生装备》
CAS
2008年第6期17-18,共2页
Chinese Medical Equipment Journal
关键词
单蒂阻隔皮瓣
模型
药物
single pedicle separartion flap
animal model
drugs
