摘要
利用菌丝生长速率法,测定了黑龙江省不同地区的10个稻瘟病菌生理小种对稻瘟灵的敏感性。在所有测试的菌株中,稻瘟灵的抑制中浓度为1.807 1 ̄4.754 2μg.ml-1,最不敏感菌株和最敏感菌株的EC50值相差2.6倍以上,表明黑龙江省的稻瘟病生理小种对稻瘟灵敏感性已存在一定程度的差异;同时,在实验室条件下比较了供试菌株在药剂选择压力(8μg.ml-1)下连续传代10次的EC50值,发现随着选择代数的增加,EC50值有增高趋势,这表明供试菌株在药剂选择压力下敏感性在逐渐降低。
The sensitivity to Isoprothiolane of 10 strains of rice blast (Magnaorthe grisea, anamorph:Pyricularia grisea) from different areas of Heilongjiang Province were measured by mycelium growth rate method. The EC50 of the strains tested to Isoprothiolane ranged from 1.8071 to 4.7542 μg·ml^-1, a difference of more than 2.6 times, showing that there existed different degrees of resistance to the pesticide among the strains. The resulted also revealed that the resistance of the resistant strains to Isoprothiolane was very, different among the generations in the experiment of continuous 10 generations of growth on YPDA medium with Isoprothiolane (8 μg·ml^-1).
出处
《北方水稻》
CAS
2008年第3期32-34,共3页
North Rice
基金
黑龙江省科技计划项目(GB06B105-2
GA06C101-01
GA06C101-03)
黑龙江省农垦总局科技项目(HNKXIV-01-04-02)资助
关键词
稻瘟病菌
稻瘟灵
敏感性
抗药风险
Magnaporthe grisea
Isoprothiolane
Sensitivity
Resistance risk