期刊文献+

液基薄层细胞学技术筛查宫颈癌临床应用研究 被引量:4

Clinical application of screening cervical cancer by thinprep cytology test
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摘要 目的应用液基薄层细胞学技术筛查女性宫颈癌,并对部分患者检测人乳头状瘤病毒(HPV)-16、-18型。方法采用液基薄层细胞学技术对医院妇科门诊患者及妇科体检者651例进行宫颈癌筛查,并采用荧光定量PCR对28例细胞学阴性者和13例低级别病变以上患者检测HPV-16、-18型。结果651例样本检出低级别病变11例,高级别病变2例,检出率分别为1.69%和0.31%,总检出率为2.0%;28例细胞学阴性者检出HPV-16、-18型3例,感染率为7.14%;13例低级别以上患者检出HPV-16、-18型6例,感染率为46.15%,感染率差异具有统计学意义。结论液基薄层细胞学技术简便实用,适宜于女性宫颈癌筛查;联合HPV-16、-18型检测更有意义。 Objective To screen cervical cancer by thinprep cytology test (TCT) and detect human papilloma virus (HPV)-16, -18 in some patients. Methods TCT was applied to screening cervical cancer in 651 cases of gynecology outpatients and healthy examination people, while real-time fluorescence quantitive PCR was adopted to detect HPV-16 and -18 in 28 cases of subjects with normal cytology and 13 cases of patients with low grade or above lesion. Results In 651 cases of samples, 11 cases (1.69%) of low grade and 2 cases (0.31%) of high grade squamous intraepithelial lesion were detected with TCT, and the total detection rate was 2.0%. The detected cases (%) of HPV-16 and- 18 were 3 (7.14%) in 28 cases of cytology negative women, and 6 (46.15%) in 13 patients with low grade or above lesion. The difference of infection rate was statistically significant. Conclusion Thinprep cytology test is simple and practical, suitable for screening of cervical cancer. It is more significant to detect HPV-16 and -18 simultaneously.
出处 《国际检验医学杂志》 CAS 2008年第6期504-505,共2页 International Journal of Laboratory Medicine
关键词 细胞学技术 宫颈肿瘤 乳头状瘤病毒科 普查 Cytological Techniques Uterine Cervical Neoplasms Papillomaviridae Mass Screening
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参考文献7

  • 1王莹,卞美璐.液基薄层宫颈细胞学[M].北京:科学技术文献出版社,2004:12.
  • 2Solomon D, Davey D, Kurman R, et al. The 2001 Bethesda System: terminology for reporting results of cervical cytology [J]. JAMA, 2002, 287(16):2 114-2 119.
  • 3Wright TC Jr, Cox JT, Massad LS, et al. 2001 Consensus Guidelines for the management of women with cervical cytologi cal abnormalities [J]. JAMA, 2002, 287(16):2 120-2 129.
  • 4Burd EM. Human papillomavirus and cervical cancer [J]. Clin Microbiol Rev, 2003, 16(1): 1-17.
  • 5Coste J, Cochand-Priollet B, de Cremoux P, et al. Cross sectional study of conventional cervical smear, monolayer cytology, and human papillomavirus DNA testing for cervical cancer screening [J]. BMJ, 2003, 326(7 392): 733.
  • 6洪蝶(综述,陈怀增(综述),谢幸(审校),吕卫国(审校).人乳头瘤病毒基因型检测方法的研究进展[J].国际检验医学杂志,2006,27(1):58-60. 被引量:9
  • 7林广玲,翁建盛,林小荣,陈林兴,林少晖,林美珊,卓静,陈慎仁.基因芯片人乳头瘤病毒分型在妇女下生殖道病变中的应用[J].国际检验医学杂志,2007,28(4):292-294. 被引量:3

二级参考文献21

  • 1黄婴,吴令英.HPV在宫颈病变中的临床意义[J].癌症进展,2004,2(5):331-338. 被引量:17
  • 2李季,曹文苓,颜景兰,黎小东,关艳冰,田广南.宫颈糜烂患者HPVs潜伏感染研究[J].中国临床医学,2005,12(1):127-128. 被引量:5
  • 3刘水平,蒋海鹰,郑晖,颜亚晖,冯德云.高危型HPV在宫颈癌发生中的作用[J].实用预防医学,2005,12(5):1023-1024. 被引量:5
  • 4Chan SY,Delius H,Halpern AL,et al.Analysis of genomic sequences of 95 papillomavirus types:uniting typing,phylogeny,and taxonomy.J Virol,1995,69(5):3074-3083.
  • 5Hesselink AT,van-den-Brule AJ,Brink AA,et al.Comparison of hybrid capture 2 with in situ hybridization for the detection of highrisk human papillomavirus in liquid based cervical samples.Cancer,2004,102(1):11-18.
  • 6Player AN,Shen LP,Kenny D,et al.Single-copy gene detection using branched DNA (bDNA) in situ hybridization.J Histochem Cytochem,2001,49(5):603-612.
  • 7Pretet JL,Dalstein V,Monnier-Benoit S,et al.High risk HPV load estimated by Hybrid Capture Ⅱ correlates with HPV16 load measured by real-time PCR in cervical smears of HPV16-infected women.J Clin Virol,2004,31(2):140-147.
  • 8Federschneider JM,Yuan L,Brodsky J,et al.The borderline or weakly positive Hybrid Capture Ⅱ HPV test:a statistical and comparative (PCR) analysis.Am J Obstet Gynecol,2004,191(3):757-761.
  • 9Petry KU,Menton S,Menton M,et al.Inclusion of HPV testing in routine cervical cancer screening for women above 29 years in Germany:results for 8466 patients.Br J Cancer,2003,88(10):1570-1577.
  • 10Castle PE,Schiffman M,Burk RD,et al.Restricted cross-reactivity of hybrid capture 2 with nononcogenic human papillomavirus types.Cancer Epidemiol Biomarkers Prev,2002,11 (11):1394-1399.

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