摘要
目的观察肾俞、百会穴针刺预处理对脑缺血再灌注模型大鼠不同时间段血清皮质酮水平的影响。方法雌性W istar大鼠160只,随机分成正常对照组、针刺预处理组、模型组和针刺预处理模型组,每组40只。针刺预处理:对双侧肾俞、百会穴进行针刺;造模:以颈动脉引流法行全脑缺血再灌注造模,术后/针后分0.5h、3h、6h、24h、48h 5个时间段眼球取血,制备血清,比色法检测皮质酮含量。结果针刺预处理组与正常对照组比较,0.5h血清皮质酮出现短暂升高(P<0.05),之后快速回落至正常水平;脑缺血再灌注模型大鼠血清皮质酮含量在再灌注0.5h急剧升高,再灌注6h达高峰(P<0.05),24h后逐渐回落,但仍高于其它各组相应时间段(P<0.05);针刺预处理模型组与模型组比较,各时段均降低,尤以6h最为显著(P<0.05)。结论肾俞、百会穴针刺预处理对脑缺血再灌注后血清皮质酮异常波动具有显著的调控作用。
Objective： To observe the influence of electroacupuncture on the levels of corticosterone in blood serum in rats with temporary cerebral ischemia -reperfusion. Method： 160 female Wistar rats were randomly divided into 4 groups： normal control group （ n = 40）, electroacupuncture preconditioning control group （ n = 40）, cerebral ischemia - reperfusion group （n = 40）, cerebal ischeinia -reperfusion and electroacupuncture preconditioning group （n = 40）. The cerebral ischemia/reperfusion injury model was established by using common carotid artery blood drainage method in rats. Blood serum was obtained from eyeballs. The levels of corticosterone were detected by enzyme - linked immunosorbent assay. Results： Acupuncture preconditioning of Shenshu and Baihui had significantly influence on the levels of corticosterone during＇ischemia/reperfusion. It cut down the levels of corticosterone in every phase of reperfusion after ischemia （ P 〈 0.05 ）. Conclusion ： The effect of acupuncture preconditioning of Shenshu and Baihui on the corticosterone levels during ischemia/reperfusion in rats might be one of the important bases for combating the injury induced by temporary cerebral ischemia/reperfusion.
出处
《湖北中医学院学报》
2008年第2期9-11,共3页
Journal of Hubei College of Traditional Chinese Medicine
关键词
脑缺血再灌注损伤
皮质酮
针刺预处理
cerebral ischemia/reperfusion injury
corticosterone
electroacupuncture preconditioning
