摘要
对扁蓿豆种质资源进行遗传多样性和亲缘关系分析，为育种者选择亲本和种质资源的开发提供理论依据。采用微卫星分子标记对来自内蒙古野生扁蓿豆种质资源50份材料进行鉴定。用8份扁蓿豆基因组DNA从89对截形苜蓿SSR引物中鉴定筛选出扩增带单一、稳定清晰且多态性强的18对引物。用这18对引物，对扁蓿豆材料的DNA进行SSR扩增，以研究其遗传多态性。结果共检测到109个等位位点，平均每对引物扩增出6．1个等位位点。6对SSR引物B14803，MTIC272，MAL369471，MTIC237，MTIC188和MTIC27对于检测扁蓿豆遗传变异最有效。供试材料间遗传距离介于0．0236～0．8075，平均遗传距离为0．1778。聚类分析构建了亲缘关系树状图，大致分为9大类。
Eighteen reproducible and informative SSR primer pairs were selected from the 89 pairs of primers of Medicago truncatula. In 8 genome DNA of Medicago ruthenica. And a representative set of 50 wild Medicago ruthenica genetic resources from Inner Mongolian were analyzed by SSR methodology using the informative primer paris for their diversity of genetic .The results showed a total of 109 alleles were detected with an average of 6.1 alleles per SSR primer pair. According to integrated evaluation, 6 SSR primer pair, BI4BO3, MTIC272, MAL369471, MTIC237, MTIC188 and MTIC27 were most effective for genetic diversity studies on Medicago ruthenica. The genetic distances among the germplasms ranged between 0.023 6 and 0.807 5 with their average at 0.177 8. By cluster analysis with sum of squares for microsatellite showed that 50 accessions can be divided into 9 groups with different characteristics which could afford reference in deeperresearch on Medicago ruthenica.
出处
《华北农学报》
CSCD
北大核心
2008年第3期67-71,共5页
Acta Agriculturae Boreali-Sinica
基金
国家科技基础条件平台建设项目(2006DKA21001-26)
农业部作物种质资源保护项目(NB307-2130135-42)
关键词
扁蓿豆
微卫星标记
多态性聚类分析
Medicago ruthenica
Microsatellite
Polymorphism
Cluster analysis
