摘要
在叶面喷施植物生长调节剂的情况下,比较了大豆叶片中保护酶活性的差异,研究了喷药后对叶片同化物代谢的调控效应,为大豆鼓粒(R5)后期抗衰老和生理代谢功能的提高提供参考。试验于2006年在大田条件下进行,为了避免遗传因子的影响,本研究只选择垦农4号品种为材料,研究对其喷药后叶片内保护酶活性和同化物代谢的变化情况。结果表明:随着喷药时间的延续,SOD模拟物(SODM)、氯化胆碱(Cc)和2-N,N-二乙氨基乙基己酸酯(DTA-6)提高了叶片中的SOD和POD活性。其中SOD活性以DTA-6为最高,SODM次之;POD活性则以SODM调节剂的为最高,调节剂Cc次之。此外,DTA-6和SODM在一定程度上还提高了叶片中的CAT活性、可溶性蛋白质、可溶性糖、硝态氮以及游离氨基酸含量,减缓了MDA含量的升高,而调节剂Cc则表现不明显。综合分析表明,叶面喷施DTA-6和SODM,维持了叶片保护酶和同化物的正常生理代谢功能,有效的提高了叶片抗氧化能力和控制了叶片的衰老进程。
The aim of this experiment was to discover difference of protective enzyme activity and study effects of metabolism assimilation in soybean leaves by spraying different plant growth regulators（PGRs）. It was also to provide references for anti-aging and increasing function of metabolism assimilation in the pod filling（ R5 ）stage. Field experiments was conducted the effects of different PGRs on metabolism assimilation and activity of protective enzyme , and Kennong4 variety was selected to avoid the impact of genetic factors in 2006. The results showed that three regulators（ SOD simulation material（SODM）, Choline chloride （Cc）and Diethyl aminoethyl hexanoate （DTA-6）increased SOD and POD activity with a continuation of spraying time in soybean leaves. SOD activity of DTA-6 was the highest, followed by SODM ; POD activity of SODM was the highest, next came Cc. In addition, SODM and DTA-6 also increased the CAT activity of leaves at a certain extent, enhanced soluble proteins,soluble sugar, as well as to nitrate content of free amino acids,and slowed down the increase of MDA. But Cc was not obvious. The above results indicated that it is effective to enhance the antioxidant capacity and slow down the senescence of its leaves, and maintaining physiological assimilation function of its metabolism assimilation and activity of protective enzyme in soybean leaves.
出处
《华北农学报》
CSCD
北大核心
2008年第3期106-111,共6页
Acta Agriculturae Boreali-Sinica
基金
国家科技支撑计划项目(2006BAD21B01)
黑龙江省"十一五"科技攻关项目(GA06B101-1-1)
关键词
植物生长调节剂
大豆
保护酶
同化物代谢
Plant growth regulators
Soybean
Protective enzyme
Metabolism assimilation
