摘要
目的通过测定小鼠低氧暴露后脑组织水、钠、钙含量,观察高压氧(HBO)预处理对小鼠低氧后脑水肿预防作用,通过测定小鼠脑组织伊文思蓝(EB)渗透量,观察对小鼠血脑屏障的影响。方法48只健康雄性昆明小白鼠,重量22～25(22.35±2.31)g,随机均分为高压氧暴露(HBO)组、高压空气(HBA)组、常压空气(NBA)组和空白对照(BC)组。HBO 组、HBA 组、NBA 组分别暴露于各自的气体环境中,每日暴露2次连续3 d,末次暴露结束后24 h,3组动物连续低氧暴露12 h 后出舱。测定小鼠脑组织水、钠、钙含量及 EB 渗透量。组织含水量测定采用分析天平脑组织干、湿重量比较法,钠、钙含量测定采用脑组织湿式灰化和原子吸收分光光谱法,脑组织 EB 含量测定采用甲酰胺法。结果 HBA 脑组织含水量与 NBA 组差异无统计学意义;HBO 组与 HBA 组相比,HBO 组脑组织含水量、钠、钙含量及 EB 含量均较低,差异有统计学意义(P<0.01)。结论高压氧预处理可预防小鼠低氧后脑水肿的发生,而预处理时单纯压力增高不能减轻低氧后脑水肿。
Objective Water content, total natrium and calcium content of mice brain were detected to evaluate prevention effect of hyperbaric oxygen （HBO） exposure on the mice brain edema. Evans blue content of mice brain tissue was detected to investigate the effects of HBO on brain edema. Methods Forty-eight male Kunming mice with weight of 22-25 （22. 5±2.31）g were randomly divided into 4 groups：HBO group, hyperbaric air （HBA） group, normal baric air （NBA） group, and blank control （BC） group. HBO group, HBA group and NBA group were exposed to designed air environment respectively, twice daily for 3 successive days. Twenty-four hours after the last exposure and under same situation, these 3 groups were exposed to hypomia for 12 hours. Water content of mice brain tissue were detected by means of analytic balance, and dry and wet weight were compared. Measurement of total natrium, total calcium adopted wet calcination of brain tissue and atomic absorption spectroscopical spectroscopy. Measurement of Evans blue （EB） content adopted formamide method. Results Compared with NBA group, water content of brain tissue had no significant statistical difference in HBA group. Compared with HBA group, water content, total natrium, total calcium and EB content of brain tissue were significantly reduced （P〈0. 01）. Conclusions HBO preconditioning can prevent brain edema causing by acute hypoxia, but increasing pressure alone during air preconditioning can not prevent brain edema.
出处
《中华航空航天医学杂志》
CSCD
2007年第4期251-254,共4页
Chinese Journal of Aerospace Medicine
关键词
高压氧
低氧
脑水肿
Hyperbaric oxygenation
Anoxia
Brain edema
