期刊文献+

兔慢性心力衰竭心房肌细胞钠离子通道重构和分子机制及厄贝沙坦的干预 被引量:3

I_(Na) remodeling and its molecular mechanisms of atrial myocytes in rabbit with chronic heart failure and effects of irbesartan on ionic remodeling
下载PDF
导出
摘要 目的观察慢性心力衰竭(简称心衰)兔心房肌细胞钠离子通道电流的变化及分子机制和厄贝沙坦的干预作用,探讨心衰房性心律失常及厄贝沙坦抑制心律失常的可能机制。方法90只兔随机分为假手术组、心衰组、厄贝沙坦组。通过结扎左室支建立兔心衰模型,厄贝沙坦组于术后第2天行厄贝沙坦干预。用全细胞膜片钳记录兔心房肌细胞钠通道电流的变化;用定量聚合酶链式反应(PCR)方法测定兔心房肌细胞钠通道α亚单位mRNA表达。结果兔心房肌细胞钠离子通道峰电流密度心衰组和厄贝沙坦组低于假手术组(-22.04±1.94pA/pF,-26.24±-2.36pA/pFvs-32.22±-2.22pA/pF),厄贝沙坦组高于心衰组。假手术组、心衰组、厄贝沙坦组钠离子通道α亚单位mRNA分别为1.7±0.35(×10-1),1.1±0.22(×10-1),1.24±0.31(×10-1)。结论兔慢性心衰心房肌细胞钠离子通道电流下调,其机制之一可能与通道α亚单位mRNA表达的减少有关,而厄贝沙坦则可抑制其下调。 Objective To explore the possible mechanisms for the atrial arrhythmia in chronic heart failure(CHF) and for antiarrhythmic effects of irbesartan by observating the change of atrial myocyte INa and INa αsubunit mRNA expression. Methods A total of 90 rabbits were randomly divided into sham-operated group,CHF group and irbesartan group. The CHF rabbit model was established by ligation of ventricular branch of the left coronary arteries. The INa and the INa current densities of left atrial myocytes in three groups were recorded with whole-cell patch clamp technique. The messenger ribonucleic acid(mRNA) of atrial sodium channel α subunit were measured by real-time reverse transcription-polymerase chain reaction. Results The peak INa current densities of atrial myocytes in CHF group and irbesartan group decreased when compared with sham-operated group( - 22.04 ± 1.94 pA/pF, - 26.24 ± - 2.36 pA/pF vs - 32.22 ±- 2.22 pA/pF), irbesartan group increased compared with CHF group. The INa α subunit mRNA expression of atrial myocytes in sham-operated group,CHF group and irbesartan group were 1.7 ±0.35( ×10^-1) ,1.1 ±0.22(×10^-1) ,1.24 ±0.31( × 10^-1). Conclusion The INa current density of left atrial myocytes reduced in CHF; The down-regulation of sodium channel α subunit mRNA level might be one of major mechanisms of down-regulation of INa current density. Irbesartan could prevent the remodeling of INa of atrial myocte with CHF.
出处 《中国心脏起搏与心电生理杂志》 2008年第3期248-251,共4页 Chinese Journal of Cardiac Pacing and Electrophysiology
关键词 心血管病学 慢性心力衰竭 心房肌细胞 钠通道 钠通道α亚单位 厄贝沙坦 Cardiology Chronic heart failure Atrial myocytes INa INaα subunit Irbesartan
  • 相关文献

参考文献11

  • 1Nattel S, Maguy A, Bouter SL, et al. Arrhythmogenic ion-channel remodeling in the heart : heart failure, myocardial infarction, and atrial fibrillation[J ]. Physiol rev ,2007,87:425
  • 2Madrid AH, Marin IM, Cervantes CE, et al. Prevention of recurrences in patients with lone atrial fibrillation. The dose-dependent effect of angiotensin Ⅱ receptor blockers. J[J]. Renin Angiotensin Aldosterone Syst ,2004,5 ( 3 ) : 114
  • 3Mauric PY, Marjorie B, Stuart MC. Comparison of in vivo and in vitro hacmodynamic function in experimental heart failure : use of cchocardiography[ J]. Cardio Res, 1996, 31:873
  • 4罗骏,杨向军,李红霞,蒋彬,蒋文平.厄贝沙坦对兔心力衰竭心房重构的影响[J].苏州大学学报(医学版),2006,26(4):550-552. 被引量:1
  • 5Yue L, Melnyk P, Gaspo R, et al. Molecular mechanisms underlying ionic remodeling in a dog model of atrial fibrillation [ J ]. Circ Res, 1999,84:776
  • 6Gaspo R, Bosch RF, Talajic M, et al. Functional mechanisms underlying tachycardia-induced sustained atrial fibrillation in a chronic dog model[ J]. Circulation, 1997,96:4027
  • 7Sanders P, Morton JB, Davidson NC, et al. Electrical remodeling of the atria in congestive heart failure: electrophysiological and electroanatomic mapping in humans [ J]. Epub, 2003,108 (12) : 1461
  • 8Fukunami M, Yamada T, Ohmori M, et al. Detection of patients at risk for paroxysmal atrial fibrillation during sinus rhythm by P wavetriggered signal-averaged electrocardiogram[J]. Circulation,2001,104:684
  • 9Li D, Shinagawa K, Pang L, et al. Effects of angiotensin converting enzyme inhibition on the development of atrial fibrillation substrate in dogs with ventricular tachycardia induced congestive heart failure [J] .Circulation,2001,104 : 2608
  • 10Goette A, Lendeckel U, Klein HU. Signal transduction systems and atrial fibrillation[ J]. Cardiovasc Res ,2002,54:247

二级参考文献7

  • 1Mauric PY,Marjorie B,Stuart MC.Comparison of in vivo and in vitro haemodynamic function in experimental heart failure:use of echocardiography[J].Cardio Res,1996,31:873-881.
  • 2ISIS-4 (fourth International Study of Infarct Survival) Collaborative Group.ISIS-4:a randomised factorial trial assessing early oral captopril,oral mononitrate and intravenous magnesium sulphate in over 58050 patients with suspected acute myocardial infarction[J].Lancet,1995,345:669-685.
  • 3Richer C,Fornes P,Cazarbon C,et al.Effects of long term angiotensin Ⅱ AT1 recepter blockade on survival,hemodynamics and cardiac remodeling in chronic heart failure in rats[J].Cardiovasc Res,1999,41(1):100-108.
  • 4Michel JB,Mercadier JJ,Galen FX,et al.Urinary cyclic guanosine monophosphate as an indicator of expe-rimental congestive heart failure in rats[J].Cardiovasc Res,1990,24:946-952.
  • 5Li D,Shinagawa K,Pang L,et al.Effects of angiotensin converting enzyme inhibition on the development of atrial fibrillation substrate in dogs with ventricular tachycardia induced congestive heart failure[J].Circulation,2001,104:2608-2614.
  • 6Goette A,Lendeckel U,Klein HU.Signal transduction systems and atrial fibrillation[J].Cardiovasc Res,2002,54:247-258.
  • 7Neuberger HR,Schotten U,Blaauw Y,et al.Chronic atrial dilation,electrical remodeling,and atrial fibrillation in the goat[J].J Am Coll Cardiol,2006,47(3):644-653.

同被引文献12

引证文献3

二级引证文献47

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部