摘要
以新疆野苹果(Malus sieversii(Lebed.)M.Roem.)无菌试管苗为试材,对其离体茎尖玻璃化超低温保存的影响因素进行研究。结果表明,新疆野苹果茎尖在含有5%二甲基亚砜(DMSO)的0.4mol/L蔗糖培养基上预培养3 d,60%玻璃化溶液(PVS2)中室温装载30m in,PVS20℃下处理40m in,经液氮保存至少24h后,转入继代培养基上再培养,成活率和再生率分别为93.3%和86.7%。再生植株生长和分化正常;同时对再生植株进行SSR标记检测,未发现超低温保存前后的DNA谱带存在差异。
Experiments were conducted about tissue culture systems and cryopreservation on Xinjiang wild apple（Malus sieversii （Lebed.） M.Roem.）. A suitable procedure was established as follows： four weeks after subculture, shoot-tips about 2 - 3 cm in length were precultured for 3 days on MS medium supplemented with 0. 4 mol /L sucroseand 5% DMSO, and pretreated in 60% plant vitrification solution （PVS2） for 30 minutes at room temperature, then followed by dehydration with PVS2 for 40 minutes at O℃ and put into liquid nitrogen for 24 hours. The survival and regeneration rate of shoot tips treated in this way was 93.3% and 86.7% respectively. The plantlets could nomally grow and differentiation. The plantlets were subjected to genetic analysis using SSR molecular marker, and the genetic difference was not found.
出处
《植物遗传资源学报》
CAS
CSCD
2008年第2期243-247,共5页
Journal of Plant Genetic Resources
基金
国家自然科学基金项目(30471196)
国家“863”计划重点项目(2006AA100108)
山东农业良种产业化项目
