摘要
目的探讨MMP-3基因单核苷酸多态性（SNP）与冠心病患者冠状动脉粥样硬化程度间的相关性。方法入选经冠状动脉造影证实的汉族冠心病患者1371例及健康对照695例，选择MMP-3-16125A／6A、-376C／G、Glu45Lys等SNPs位点，采用聚合酶链式反应-限制性内切酶片段长度多态性（PCR-RFLP）方法确定基因型。X^2检验用于单因素分析时检验SNP位点与冠状动脉粥样硬化程度间的关联。结果（1）-16125A／6A多态性位点在冠心病患者单支病变、双支病变、三支病变组中5A等位基因频率分别是0．185，0．183，0．152；-376C／G多态性位点在以上三组中一376G等位基因频率分别是0．329，0．326，0．325；Glu45Lys多态性位点在以上三组中45Lys等位基因频率分别是0．423，0．417，0．405。（2）-16125A／6A多态性位点5A等位基因频率在三支病变组中明显低于单支病变组（OR=0．74，P=0．04）；相对于6A／6A基因型，含有5A等位基因的纯合子及杂合子频率在三支病变组明显低于单支病变组（OR=0．74，P=0．04）。结论中国汉族冠心病患者中MMP-3基因-16125A／6A多态性位点可能与冠心病患者冠状动脉粥样硬化程度相关，5A等位基因可能对冠状动脉粥样硬化的进展有保护作用。
Objective To investigate the association between the severity of coronary arteries in patients with coronary artery disease and the single nucleotide polymorphisms of MMP-3 gene. Methods One thousand and three hundred seventy-one patients with coronary artery disease （CAD） diagnosed by coronary angiography and six hundred and ninety-five healthy controls without CAD were enrolled in this study. The SNPs of - 1612 5A/6A, -376C/G, Glu45Lys of MMP-3 were genotyped by restriction fragment length polymorphism analysis （RFLP） in all subjects. Univariate analysis was applied to measure the association of the single nucleotide polymorphisms with the severity of coronary arteries. Results The minor allele frequency of - 1612 5A/6A was 0. 189, 0. 185, 0. 183 and 0. 152（P 〈0. 05 vs. non-CAD control and single stenosis）, the minor allele frequency of -376C/G was 0. 311, 0. 329, 0. 326 and 0. 325, and the minor allele frequency of Glu45Lys was 0. 367, 0. 423, 0. 417 and 0. 405 in non-CAD control, CAD patients with single, two and three vessels stenosis, respectively. 5A allele frequency is significant lower in the group with three vessels stenosis than in non-CAD control and CAD patients with single vessel stenosis （ OR = 0. 74, P =0. 04）. The 5A/SA and 5A/6A genotypes frequency is significant lower in the group with three vessels stenosis than in the non-CAD group and CAD patients with single vessel stenosis （ OR = 0. 74, P = 0. 04）. Conclusions The single nucleotide polymorphism of - 1612 5A/6A of MMP-3 gene may be associated with the severity of coronary atheroslerosis in the Chinese Han patients with coronary artery disease, and the 5A allele might therefore, play a protective role on the progression of coronary atherosclerosis.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2008年第6期501-505,共5页
Chinese Journal of Cardiology
关键词
基质金属蛋白酶-3
冠状动脉硬化
多态性
单核苷酸
Matrix metalloproteinase-3
Coronary atherosclerosis
Polymorphism, single nucleotide
