摘要
利用农杆菌介导法将甜椒热激蛋白基因转化番茄,Northern和Western杂交表明CaHSP18在番茄植株中表达,获得转CaHSP18的番茄植株。Northern杂交显示,CaHSP18基因受低温诱导,表达量随低温处理时间的延长而增加,6h时表达量最高。低温胁迫导致野生型和转基因番茄植株的相对电导率升高,光系统Ⅱ(PSII)最大光化学效率(F_v/F_m)和放氧速率下降,但转基因番茄植物维持较低的膜透性,较高的F_v/F_m和放氧速率。这些显示,在番茄植株中CaHSP18表达后耐冷性有提高。
Sweet pepper CaHSP18 was introduced into tomato by the A. tumefaciens-mediated method. Northem and Western blot showed that CaHSP18 gene was recombined into the genome of tomato in some tomato transgenic lines. Northern blot showed that the expression of CaHSP18 was induced by low temperature stress, and the highest mRNA level was observed after 6 h under chilling stress. Chilling stress caused the increase in relative electronic conductance of membrane, and the decrease in maximal fluorescence efficiency of PSII （Fv/ Fm） and oxygen evolution rate of both transgenic and wild tomato plants. However, transgenic tomato plants with expression of CaHSP18 could maintain the low relative conductance of membranes, higher Fv/Fm and oxygen evolution rate. It indicated that expression of CattSP18 enhanced the tolerance of transgentic tomato to chilling stress.
出处
《植物生理学通讯》
CSCD
北大核心
2008年第3期409-412,共4页
Plant Physiology Communications
基金
山东省自然科学基金(Y2007D50)。
