摘要
目的探讨血管生成拟态(vasculogenic mimicry,VM)和低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)在肺癌中的表达及意义。方法收集64例人肺癌石蜡包埋样本,进行PAS及CD34免疫组化双重染色,检测肺癌中是否存在VM;采用免疫组化SP法检测HIF-1α蛋白在肺癌组织中的表达特点。结果肺癌中VM和HIF-1α表达阳性率分别为37.50%(24/64)、57.81%(37/64),显著高于癌旁正常肺组织(0/15)(P<0.05)。VM在低分化肺癌组(56.67%)高于高-中分化肺癌组(20.59%),与TNM分期、淋巴结转移有关(P<0.05)。HIF-1α的表达也与淋巴结转移及临床分期有关(P<0.05);VM与HIF-1α的表达呈正相关(r=0.335,P<0.05)。结论肺癌中存在VM,HIF-1α高表达可能促进VM。VM与HIF-1α高表达可能是肺癌浸润、转移重要生物学标志。VM与HIF-1α联合检测对肺癌的进展及预后判断有重要意义。
Purpose To explore whether vasculogenic mimicry （VM） exists in lung cancer or not and to elucidate the relationship between the expression of HIF-1α and VM. Methods 64 lung cancer resection specimens were collected. The presence of VM was observed by immunohistochemical and histochemical double staining of CD34 and PAS. The expression of HIF-1α protein was detected by immunohistochemical staining. Results In 64 cases of lung cancer, 24 （ 37.50% ） exhibited evidence of VM and 37 （ 57.81% ） were positive for HIF-1α, which were significantly higher than that of normal lung tissue （ P 〈 0. 05 ）. VM in lowly differentiated lung cancer （56. 67% ） was higher than that in moderately to well differentiated （20. 59% ） and it was significantly related to TNM stage and lymph node metastasis （P 〈 0. 05 ）. The expression of HIF-1α protein was also related to the TNM stage and lymph node metastasis （ P 〈 0.05 ）. There was a positive association between the expression of HIF-1α and VM （ r = 0. 335, P 〈 0. 05 ）. Conclusions VM exists in lung cancer,overexpression of HIF-1α may induce the formation of VM channels. VM and over-expression of HIF-1α may be important biological markers for malignant transformation ,invasion and metastasis of lung cancer. The combined detection of VM and HIF-1α has an important role in predicting the progression and prognosis of lung cancer.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2008年第3期269-272,共4页
Chinese Journal of Clinical and Experimental Pathology
关键词
肺肿瘤
血管生成拟态
低氧诱导因子-1Α
lung neoplasms
vasculogenic mimicry
hypoxia-inducible factor-1 alpha
