摘要
采用反相高效液相色谱测定不同提取条件下柑桔皮中橙皮苷和柚皮苷的含量,同时采用光电二极管阵列检测器检测两组分的紫外光谱。色谱柱为ODS C18柱(150mm×4.6mm,5μm),流动相为甲醇-水(体积比为40/60),流速为1.0mL/min,检测波长270nm,柱温25℃。橙皮苷和柚皮苷的线性范围分别为0.007168～0.28g/L和0.012288～0.48g/L;二者含量分别为17.5～21.9mg/g(RSD=0.77%～1.86%)和2.14～2.78mg/g(RSD=0.98%～1.59%);其平均回收率分别为98.5%～99.4%(RSD=0.69%～1.78%)和99.3%～101.1%(RSD=0.43%～1.45%);检出限分别为0.28μg和0.16μg。该法简便、准确、重现性好,线性范围宽,适于对含有两组分的植物进行质量评价。
An RP-HPLC method was established for the determination of hesperidin and naringin in the citrus peel under different extracted conditions, and at the same time UV spectrum of two components was detected by means of Photodiode Array Detector （PAD）. Hesperidin and naringin were separated on an ODS C18 column （150mm×4.6mm,5μm） with methanol-water （volume ratio of 40/60） as the mobile phase and detected at 270nm. The flow rate was 1.0mL/min. The column temperature was set at 25℃. The calibration curves were linear in the ranges of 0.007168-0.28g/L and 0.012288-0.48g/L for hesperidin and naringin respectively. Determination of hesperidin was 17.5-21.9mg/g （ RSD = 0.77%- 1.86% ） and naringin determination was 2.14-2.78mg/g （ RSD = 0.98%-1.59%）. The average recoveries were 98.5%-99.4% （ RSD = 0.69%-1.78%） for hesperidin and 99.3%- 101.1% （RSD = 0.43%-1.45%） for naringin, and the detection limits were 0.28μg and 0.16μg respectively. This method was simple, accurate, reproducible and had wider linear range, which could be adapted to evaluate the quality of plant that contains two component.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第6期288-290,共3页
Science and Technology of Food Industry
基金
国家高技术研究发展计划(2007AA10Z307)
湖南省“十一五”重大科技专项(2006NK1002)资助
