摘要
目的:研究染料木素自微乳在大鼠的在体肠吸收机制。方法:采用大鼠在体肠回流装置,UV,HPLC分别测定酚红和染料木素的含量。考察药物浓度、pH、不同肠段及P-糖蛋白(P-gp)抑制剂维拉帕米对染料木素吸收的影响。结果:染料木素自微乳在药物浓度为0.05~0.5 g·L^(-1)和pH为5.4~7.8时小肠吸收速率常数K_a均无统计差异;各肠段的吸收速率常数K_a空肠>回肠>十二指肠>结肠,空肠与其他肠段有统计差异(P<0.05);维拉帕米显著增加染料木素的肠吸收(P<0.05)。结论:染料木素自微乳在大鼠肠道的吸收呈一级动力学过程,吸收机制为被动扩散,在整个肠段都有吸收,最佳吸收部位在空肠。药物吸收受P-糖蛋白外排影响,而pH对药物吸收无显著影响。
Objective: To investigate the absorption mechanism of genistein self-microemulsifying system in rat intestines. Method: The concentrations of phenol red and genistein by in situ perfusion in rats were determined by UV and HPLC, respectively. The effects of drug concentrations, pH, various intestinal segments and P-glycoprotein (P-gp) inhibitor verapamil on the absorption had been studied. Result: The absorption rate constant (Ka ) of genistein had no significant difference at concentrations of 0. 05 ~ 0. 5 mg · mL^-1 and pH of 5.4 ~ 7.8 in perfusion. It was Ka of jejunum 〉 ileum 〉 duodenum 〉 colon. The absorption of genistein in jejunum had significant difference (P 〈 0.05 ) compared with other parts of intestines. Ko was increased obviously when verapamil was coperfused with genistein ( P 〈 0. 05 ). Conclusion: The absorption of genistein seff-microemulsifying system is a first order process with passive diffusion mechanism related to P-gp efflux. It can be absorbed at all segments of rat intestine, and the jejunum is the best absorption segment, pH had no special effect on the absorption of genistein self-microemulsifying system in rat intestime.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第12期1406-1409,共4页
China Journal of Chinese Materia Medica
基金
国家“十五”科技攻关项目(2004BA721A46)
关键词
染料木素自微乳
肠吸收动力学
P-糖蛋白抑制剂
genistein self-microemulsifying system
intestinal absorption kinetics
P-glycoprotein inhibitor