摘要
研究了用气相色谱-质谱联用法，用选择离子监测技术测定卷烟主流烟气中烟草特有亚硝胺N’-亚硝基去甲基烟碱（NNN）和4-（N-亚硝基甲氨基）-1-（3-吡啶基）-1-丁酮（NNK）。在标准吸烟条件下，将烟气粒相物收集在剑桥滤片上，用柠檬酸-磷酸盐缓冲液和环己烷对粒相物共提取，提取液相继用碱性氧化铝层析柱和固相萃取法进一步纯化。选用选择离子监测技术对亚硝胺定性和定量。在进样量2μL时，方法检出限为9．83μg·L^-1（NNN）和16．92μg·L^-1（NNK），线性范围0．1～5．0mg·L^-1，相关系数分别为0．998，0．996，回收率分别为91．0％，94．8％。
A method of hyphenated GC-MS with selected ion monitoring （SIM） for the determination of specific nitrosamines of tobacco, including N＇-nitrosomor-nicotine （NNN） and 4-（N-nitroso-methylamino）-1-（3- pyrigyl）-1-butanone （NNK）, in mainstream smoke of cigarette was studied and reported in this paper. The cigarette smoking particulates were collected on a Cambridge filter with a machine under standard smoking conditions. The collected matter was co-extracted with mixture of citrate-phosphate buffer and cyclohexane and then purified on alkaline Al2O3 column and by solid-phase extraction. Identification and quantification of the analytes were performed by the selected ion monitoring technique. Detection limits of the method found were 9.83 μg·L^-1 for NNN and 16.92μg·L^-1 for NNK. Linearity was in the range of 0. 1-5.0 mg · L^-1 with correlation coefficient of 0. 998, 0.996 and recovery of 91.0%, 94. 8% respectively.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2008年第6期529-531,534,共4页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
