摘要
目的:探讨泛素降解途径蛋白Skp2和p27kip1在前列腺癌中的表达及与前列腺癌各项临床病理特征的关系,并探讨两者的相关性。方法:应用免疫组织化学法和图像分析系统研究41例前列腺癌标本、20例开放手术切除良性前列腺增生标本中Skp2和p27kip1的表达情况。分析临床病理资料与免疫组化的结果及Skp2、p27kip1之间的相关性。结果:在前列腺癌中的Skp2蛋白染色阳性率显著高于良性前列腺增生(P<0.01);前列腺癌中的p27kip1蛋白染色阳性率显著低于良性前列腺增生(P<0.01)。Skp2蛋白表达与前列腺癌术前前列腺特异性抗原(PSA)水平、肿瘤穿透前列腺被膜、肿瘤分期、病理分级均显著相关(均P<0.05)。p27kip1蛋白表达与上述临床病理特征负相关(均P<0.05)。Skp2和p27kip1蛋白表达呈负相关(r=-0.572,P<0.01)。结论:Skp2蛋白在前列腺癌中表达增加,其参与了前列腺癌的发病过程。Skp2蛋白表达与p27kip1蛋白表达之间负相关。Skp2可能成为前列腺癌治疗的一个新的靶标。
Objective:To investigate the correlation between Skp2 and p27^kip1 protein expression and the clinicopathological features of prostatic carcinoma,and study the relationships between them.Methods:Skp2 and p27^kip1 protein expression were evaluated in the tissues of 41 human prostatic carcinomas as well as 20 benign prostatic hyperplasia(BPH)using immunohistochemistry.Results:The Skp2 labeling frequency in prostatic carcinoma was significantly higher than that in BPH(P〈0.01).The p27^kip1 labeling frequency in prostatic carcinoma was significantly lower than that in BPH (P〈0.01). The Skp2 protein expression in prostatic carcinoma was positively correlated with preoperative serum prostate-specific antigen level,extraprostatic extension,clinical stage and histological grade(P〈0.05).The p27^kip1 protein expression in prostatic carcinoma were inversely correlated with preoperative serum prostate-specific antigen level,extraprostatic extension,clinical stage and histological grade(P〈0.05).Conclusion:Expression of Skp2 protein may lead to decrease in level of p27^kip1 in human prostatic carcinoma,indicating its involvement in the development of human prostatic carcinoma.In addition,Skp2 may be a new target for the treatment of prostate cancer.
出处
《中日友好医院学报》
2008年第3期160-161,164,F0003,共4页
Journal of China-Japan Friendship Hospital