摘要
目的:观察缺氧及血管紧张素Ⅱ(Ang-Ⅱ)对人大动脉血管内皮细胞(VEC)的线粒体膜电位(MMP)的影响及金钠多的保护作用。方法:建立VEC缺氧损伤模型;实验分为空白对照组、单纯缺氧组、缺氧加金钠多保护组、单纯Ang-Ⅱ作用组、Ang-Ⅱ加金钠多保护组、缺氧加Ang-Ⅱ作用组、缺氧加Ang-Ⅱ加金钠多保护组共7个组。各组细胞经过Rhodamine 123的负载后,用激光共聚焦显微镜测定VEC内Rhodamine 123的荧光强度代表MMP。结果:VEC分别经过缺氧及Ang-Ⅱ作用后,MMP显著降低(P<0.01);缺氧条件下Ang-Ⅱ引起VEC的MMP变化较单纯缺氧或单纯Ang-Ⅱ作用进一步降低(P<0.01);金钠多可抑制MMP降低,但各金钠多保护组的MMP仍显著低于空白对照组(P<0.01)。结论:缺氧及Ang-Ⅱ均可引起VEC的MMP降低,金钠多明显减轻上述影响因素的损伤、提高MMP,从而发挥对VEC的保护作用。
Objective：To study the effect of hypoxia and angiotensinⅡ（AngⅡ）on mitochondria membrane potential（MMP）in human arterial vascular endothelial cells（VEC）and the protective effect of Ginaton.Methods：Hypoxia-induced injury model of VEC was established,and then cultured VEC were divided into seven groups：control group,simple hypoxia group,hypoxia＋Ginaton group,simple AngⅡ group,AngⅡ＋Ginaton group,hypoxia＋AngⅡ group, hypoxia＋AngⅡ＋Ginaton group,which were loaded with Rhodamine 123.MMP was detected by laser confocal microscopy to see if there was any difference among these groups.Results：Compared with control group,MMP significantly decreased either in the hypoxia group or in the AngⅡ group（P〈0.01）.This change was clearer in the hypoxia＋AngⅡ group than in the other groups（P〈0.01）.MMP change could be significantly lessened by Ginaton,but MMP of each Ginaton treated group was still lower than that of the control group（P〈0.01）.Conclusion：Hypoxia or AngⅡ could significantly decrease MMP of VEC and Ginaton could increase the MMP.Ginaton has remarkably protective effect on VEC injured by hypoxia and AngⅡ.
出处
《中日友好医院学报》
2008年第3期168-170,共3页
Journal of China-Japan Friendship Hospital
基金
海军后勤科研基金(00-3321)
关键词
缺氧
血管紧张素Ⅱ
血管内皮细胞
线粒体膜电位
金钠多
hypoxia
angiotesinⅡ
vascular endothelial cells
mitochondria membrane potential
Ginaton
