摘要
以猪血球蛋白为原料,利用中性蛋白酶与风味蛋白酶对血球蛋白进行水解。在单因素试验的基础上采用响应面分析法进行双酶复合水解条件进行优化,得出双酶水解的最适工艺条件参数为:温度50℃,底物浓度12%,中性蛋白酶加量3820U/g蛋白,风味蛋白酶加量43.5LAPU/g蛋白,在未调节pH条件下酶解22.8h,此时水解度可达37.81%(预测值38.03%)。
In this trial,porcine hemocyte was taken as subatrate,response surface analysis （RSA）was conducted to obtain the optical conditions of processing through enzymatic hydrolysis of neutral protease and flavourzyme simultaneously.The results indicated that the optical technological parameter as follows ：substrate concentration 12 % ,temperature 50℃, [E/S] of neutral protease 3820 U/g protein, [E/S] of flavourzyme 43.5 LAPU/g protein,22.8 h at natrual condition.The degree of hydrolysis 37.81% by experiment（the predicted value was 38.03 %）.
出处
《中国饲料》
北大核心
2008年第10期41-44,共4页
China Feed
基金
湖北省教育厅05年攻关计划
关键词
猪血球蛋白
响应面分析法
酶水解
porcine hemocyte
response surface analysis
enzymatic hydrolysis
