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Purification and Characterization of a Proteolytic Enzyme from Fig Latex 被引量:4

Purification and Characterization of a Proteolytic Enzyme from Fig Latex
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摘要 Ficin is an important component of plants in Ficus family such as fig latex. It is of special significance in medicine and industry because it exhibits activity throughout a wide range of temperature and pH values. In this work, we purified a component of ficin from the latex homogeneity of Shandong fig trees, and the properties of the purified ficin were studied. The current findings revealed that heavy metal ions were able to inhibit ficin, while DTT, L-cysteine, and β-ME were found to promote ficin activity. It was also observed that the half life of ficin at 65 °C was longer than 1 h and the Michaelis constant(Km) for casein hydrolyzation was determined to be 1.56 mg/mL. Our study shows that this purified ficin is a cysteine protease. Ficin is an important component of plants in Ficus family such as fig latex. It is of special significance in medicine and industry because it exhibits activity throughout a wide range of temperature and pH values. In this work, we purified a component of ficin from the latex homogeneity of Shandong fig trees, and the properties of the purified ficin were studied. The current findings revealed that heavy metal ions were able to inhibit ficin, while DTT, L-cysteine, and β-ME were found to promote ficin activity. It was also observed that the half life of ficin at 65 °C was longer than 1 h and the Michaelis constant(Km) for casein hydrolyzation was determined to be 1.56 mg/mL. Our study shows that this purified ficin is a cysteine protease.
出处 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2008年第3期348-352,共5页 高等学校化学研究(英文版)
基金 the National Natural Science Foundation of China(Nos.20771030 and 20671025)
关键词 PROTEASE Fig-tree latex FICIN PURIFICATION Characterization Protease Fig-tree latex Ficin Purification Characterization
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参考文献14

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同被引文献9

  • 1Morcelle S R, Trejo S A, Canals F, et al. Funastrain c Ⅱ: a Cysteine Endopeptidase Purified from the Latex of Funastrum Clausum [J]. The Protein Journal, 2004, 23(3):205-215.
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  • 8黄晓月,毕思远,区家豪,孙力军,陈斯琪,房志家,邓旗,邓义佳,王雅玲.木瓜蛋白酶法制备抗氧化活性壳寡糖的工艺优化[J].生物学杂志,2022,39(1):104-109. 被引量:12
  • 9Jian Li,Tiantian Feng,Lin Han,Min Zhang,Tao Jiang.Fabricating biopolymer-inorganic hybrid microspheres for enzyme immobilization:Connect membrane emulsification with biomimetic mineralization[J].Particuology,2022,20(5):171-177. 被引量:1

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