摘要
精原干细胞(SSCs)是指位于睾丸生精小管基膜上既能自我更新维持自身群体数量恒定,又能定向分化形成精母细胞,最终形成精子的一类原始干细胞。其体外培养以及近年来兴起的移植、基因转染的深入研究,为探讨精子的发生机制、重建不育个体的精子发生、生产转基因动物提供了新的途径。文章综述了精原干细胞体外培养的研究现状,并对其体外的纯化、鉴定,以及未来的应用进行了介绍,旨在为精原干细胞的研究提供借鉴。
Spermatogonial stem cells were located in seminiferous tubule which not only can maintain the number of themselves but also can differentiate to spermatocyte. The continuous research in recent years, such as culture in vitro, transplantation and transgenic modification technology has presented a new method to study the spermatogenesis mechanism, rehuilting spermiotelesis in infertile individual and producing transgenic animals. This review focuses on the advance of spermatogonial stem cell culture in vitro, isolation,identification and its applications in future. It may provide the theoretical assistance to spermatogonial stem cell research.
出处
《动物医学进展》
CSCD
2008年第6期87-89,共3页
Progress In Veterinary Medicine
基金
春辉计划(A232)
国家“863”资助项目(2005AA001420)
关键词
干细胞生物学
体外培养
精原干细胞
stem cell biology
culture in vitro
spermatogonial stem cells
